Detailed information of TA system TA223332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 20837..21480
• Replicon: plasmid p2247421-T5-MAC_3
• Accession: NZ_CP086442
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-MAC

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: LNN53_RS28735
• Protein ID: WP_001044770.1
• Coordinates: 20837..21253 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LNN53_RS28740
• Protein ID: WP_001261282.1
• Coordinates: 21250..21480 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN53_RS28700 (LNN53_28705)	16379..16615	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
LNN53_RS28705 (LNN53_28710)	16612..16974	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
LNN53_RS28710 (LNN53_28715)	16992..18686	-	1695	WP_020442400.1	mercury(II) reductase	-
LNN53_RS28715 (LNN53_28720)	18738..19160	-	423	WP_001340589.1	organomercurial transporter MerC	-
LNN53_RS28720 (LNN53_28725)	19196..19306	-	111	Protein_22	mercuric transport protein periplasmic component	-
LNN53_RS28730 (LNN53_28735)	20074..20763	-	690	Protein_24	AAA family ATPase	-
LNN53_RS28735 (LNN53_28740)	20837..21253	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
LNN53_RS28740 (LNN53_28745)	21250..21480	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LNN53_RS28745 (LNN53_28750)	21437..21898	+	462	WP_072202616.1	hypothetical protein	-
LNN53_RS28750 (LNN53_28755)	22042..22392	+	351	WP_000493378.1	hypothetical protein	-
LNN53_RS28755 (LNN53_28760)	22443..23186	+	744	WP_000129823.1	hypothetical protein	-
LNN53_RS28760 (LNN53_28765)	23183..23959	+	777	WP_000015958.1	site-specific integrase	-
LNN53_RS28765 (LNN53_28770)	24017..24274	-	258	WP_000764642.1	hypothetical protein	-
LNN53_RS28770 (LNN53_28775)	24403..24507	-	105	WP_032409716.1	hypothetical protein	-
LNN53_RS28775 (LNN53_28780)	25042..25908	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-15 / blaTEM-1A / dfrA14	-	1..70475	70475

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T223332 WP_001044770.1 NZ_CP086442:c21253-20837 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T223332 NZ_CP118701:c82921-82670 [Aeromonas hydrophila]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT223332 WP_001261282.1 NZ_CP086442:c21480-21250 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT223332 NZ_CP118701:c82673-82419 [Aeromonas hydrophila]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3