Detailed information of TA system TA223331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid p2247421-T5-MAC_2
• Accession: NZ_CP086441
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-MAC

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LNN53_RS28020
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LNN53_RS28015
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN53_RS27970 (LNN53_27975)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
LNN53_RS27975 (LNN53_27980)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LNN53_RS27980 (LNN53_27985)	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
LNN53_RS27985 (LNN53_27990)	51106..51681	+	576	WP_004152103.1	hypothetical protein	-
LNN53_RS27990 (LNN53_27995)	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
LNN53_RS27995 (LNN53_28000)	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
LNN53_RS28000 (LNN53_28005)	52652..52855	+	204	WP_004152106.1	HHA domain-containing protein	-
LNN53_RS28005 (LNN53_28010)	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
LNN53_RS28010 (LNN53_28015)	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
LNN53_RS28015 (LNN53_28020)	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LNN53_RS28020 (LNN53_28025)	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LNN53_RS28025 (LNN53_28030)	54604..56007	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LNN53_RS28030 (LNN53_28035)	56036..56668	-	633	WP_001567369.1	hypothetical protein	-
LNN53_RS28035 (LNN53_28040)	56898..58244	+	1347	WP_020314316.1	ISNCY family transposase	-
LNN53_RS29610	58405..58536	+	132	WP_004218042.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSCO-1 / aac(3)-IIa / blaTEM-1B	-	1..158444	158444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T223331 WP_003026803.1 NZ_CP086441:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T223331 NZ_CP118701:c82673-82419 [Aeromonas hydrophila]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT223331 WP_003026799.1 NZ_CP086441:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT223331 NZ_CP118701:c82921-82670 [Aeromonas hydrophila]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66