Detailed information of TA system TA223328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5069216..5070897
• Replicon: chromosome
• Accession: NZ_CP086439
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-MAC

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LNN53_RS24920
• Protein ID: WP_004146663.1
• Coordinates: 5069216..5070133 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LNN53_RS24925
• Protein ID: WP_032418188.1
• Coordinates: 5070139..5070897 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN53_RS24900 (5064522)	5064522..5066042	+	1521	WP_032418186.1	sugar ABC transporter ATP-binding protein	-
LNN53_RS24905 (5066039)	5066039..5067037	+	999	WP_002885172.1	ribose ABC transporter permease	-
LNN53_RS24910 (5067068)	5067068..5068009	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LNN53_RS24915 (5068084)	5068084..5069091	+	1008	WP_032418187.1	carbohydrate kinase family protein	-
LNN53_RS24920 (5069216)	5069216..5070133	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LNN53_RS24925 (5070139)	5070139..5070897	-	759	WP_032418188.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LNN53_RS24930 (5070983)	5070983..5071711	-	729	WP_004210017.1	response regulator transcription factor	-
LNN53_RS24935 (5071984)	5071984..5074131	+	2148	WP_077598926.1	formate dehydrogenase subunit alpha	-
LNN53_RS24940 (5074128)	5074128..5074271	-	144	WP_022631365.1	hypothetical protein	-
LNN53_RS24945 (5074477)	5074477..5075163	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T223328 WP_004146663.1 NZ_CP086439:c5070133-5069216 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T223328 NZ_CP118687:4117734-4118150 [Rhizobium sp. MJ22]
ATGATCCATCTTGATACCAATGTCGCAGTCGCGCTCTTGAACGGACGGCCACGTCAAGTTCGCGAGCGCTTCGATGAGGC
GCGAAATGCAGGAACCCCGCTCGGCCTGTCAATCATCGTCTATCATGAACTGATGTATGGTGCCGCAGCCAGCGAGCGCC
GACACGCGAACGAGGAGAAGATCGCCCTGTTCATCGCTTCGGGCGGGATTTCTCTGATCGATTTCAATGAGGGCGACGCA
CAAGAAGCAGCGGATATTCGCGCCTATCTCCGGAGACAGGGCACACCGATCGGGCCGTATGATGTACTGATCGCCGCCCA
AGCGCGCCGAGCCGGCACCGTGCTGGTGACAGCCAATACAGGCGAGTTCGTCCGTGTGCCTGGCCTGCAAGTGCTCGACT
GGGCAGCAGCCTCGTAG

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28620.99 Da
• Isoelectric Point: 10.0001

>AT223328 WP_032418188.1 NZ_CP086439:c5070897-5070139 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPETLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT223328 NZ_CP118687:4117492-4117734 [Rhizobium sp. MJ22]
ATGAGCGCGACCGCCAAGCTATTCACGCATGGGCGCAGCCAGGCCGTGCGCCTGCCAAAAGAGTTCCGCTTCGAAGGCAA
GGAGGTTCGGGTCAGCCGGGTCGGCAATCGAGTCATCCTCGAACCGCTCGACGCTGACAATGCCATGCCTTGGGCAGCAA
TCGACAAGCTGGGCGACACGCCTTTCATGCCCGAAGGTCGTGAGCAACCATCGATGCCAGCCGACCGCGCCGTGTTCGAC
TGA

Structures

Toxin

• AlphaFold DB: J2XCI1