Detailed information of TA system TA223324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2797485..2799073
• Replicon: chromosome
• Accession: NZ_CP086439
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-MAC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LNN53_RS13880
• Protein ID: WP_004184027.1
• Coordinates: 2797485..2798807 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LNN53_RS13885
• Protein ID: WP_004176198.1
• Coordinates: 2798807..2799073 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN53_RS13865 (2792541)	2792541..2795042	+	2502	WP_032418977.1	fimbria/pilus outer membrane usher protein	-
LNN53_RS13870 (2795030)	2795030..2796034	+	1005	WP_016946380.1	fimbrial protein	-
LNN53_RS13875 (2796176)	2796176..2797468	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LNN53_RS13880 (2797485)	2797485..2798807	-	1323	WP_004184027.1	type II toxin-antitoxin system HipA family toxin	Toxin
LNN53_RS13885 (2798807)	2798807..2799073	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LNN53_RS13890 (2799339)	2799339..2799665	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
LNN53_RS13895 (2799662)	2799662..2800162	-	501	WP_032418978.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LNN53_RS13900 (2800140)	2800140..2801519	-	1380	WP_032418979.1	nucleobase:cation symporter-2 family protein	-
LNN53_RS13905 (2801865)	2801865..2803019	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LNN53_RS13910 (2803000)	2803000..2803923	-	924	WP_032418980.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T223324 WP_004184027.1 NZ_CP086439:c2798807-2797485 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T223324 NZ_CP118677:c1167264-1166986 [Pseudomonas juntendi]
ATGATTCAAAGCTTCAGCTGTGCCGACACGGAAGCACTATTTGTTACTGGAAAAACGCGACGTTGGTCAGATATCAAATC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCAACAGAGCTGCGAGACCTGCGATCGCCACCTGGCAACCGCCTCG
AGGCGTTGAGCGGCAACCGTGCCGGTCAGCACAGCATTCGCGTCAATGACCAATGGCGCTTGTGCTTCACCTGGACCGAC
AACGGGCCGGCCAACGTTGAAATTATCGATTACCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT223324 WP_004176198.1 NZ_CP086439:c2799073-2798807 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT223324 NZ_CP118677:c1166977-1166678 [Pseudomonas juntendi]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCCGGTGAAGTCCTGCGTGAGGACTTCCAGAAAGAAATGGGCTTCAGTGC
TGCGGCATTGGCCAGGGCGCTGGGCGTCGCCACTCCAACCGTCAACAATATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ATATGGCGCTGCGTCTGTCCATTTGCCTGGATACTACCCCCGAGTTCTGGCTCAACCTGCAGACCGCCTTCGATCTGCGC
ACCGCCGAACAGCGACACGGTGAAGAGATCATCGGGGCGGTACAGCGCCTGGTCGCCTGA