Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20832..21475 Replicon plasmid p2247421-T5-ESBL_3
Accession NZ_CP086434
Organism Klebsiella pneumoniae strain AE-2247421-T5-ESBL

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LNN54_RS28670 Protein ID WP_001044770.1
Coordinates 20832..21248 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LNN54_RS28675 Protein ID WP_001261282.1
Coordinates 21245..21475 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN54_RS28635 (LNN54_28640) 16377..16613 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
LNN54_RS28640 (LNN54_28645) 16610..16972 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
LNN54_RS28645 (LNN54_28650) 16990..18681 - 1692 WP_242914183.1 mercury(II) reductase -
LNN54_RS28650 (LNN54_28655) 18733..19155 - 423 WP_001340589.1 organomercurial transporter MerC -
LNN54_RS28655 (LNN54_28660) 19191..19301 - 111 Protein_22 mercuric transport protein periplasmic component -
LNN54_RS28665 (LNN54_28670) 20069..20758 - 690 Protein_24 AAA family ATPase -
LNN54_RS28670 (LNN54_28675) 20832..21248 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN54_RS28675 (LNN54_28680) 21245..21475 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LNN54_RS28680 (LNN54_28685) 21432..21893 + 462 WP_072202616.1 hypothetical protein -
LNN54_RS28685 (LNN54_28690) 22037..22387 + 351 WP_000493378.1 hypothetical protein -
LNN54_RS28690 (LNN54_28695) 22438..23181 + 744 WP_000129823.1 hypothetical protein -
LNN54_RS28695 (LNN54_28700) 23178..23954 + 777 WP_000015958.1 site-specific integrase -
LNN54_RS28700 (LNN54_28705) 24012..24269 - 258 WP_000764642.1 hypothetical protein -
LNN54_RS28705 (LNN54_28710) 24398..24502 - 105 WP_032409716.1 hypothetical protein -
LNN54_RS28710 (LNN54_28715) 25037..25903 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / blaTEM-1A / dfrA14 - 1..71315 71315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T223312 WP_001044770.1 NZ_CP086434:c21248-20832 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T223312 NZ_CP118641:1905806-1906393 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT223312 WP_001261282.1 NZ_CP086434:c21475-21245 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT223312 NZ_CP118641:1906460-1907314 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTACCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTAGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References