Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5386409..5387034 Replicon chromosome
Accession NZ_CP086431
Organism Klebsiella pneumoniae strain AE-2247421-T5-ESBL

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN54_RS26395 Protein ID WP_002882817.1
Coordinates 5386409..5386792 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN54_RS26400 Protein ID WP_004150355.1
Coordinates 5386792..5387034 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN54_RS26380 (5383775) 5383775..5384677 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN54_RS26385 (5384674) 5384674..5385309 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN54_RS26390 (5385306) 5385306..5386235 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN54_RS26395 (5386409) 5386409..5386792 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN54_RS26400 (5386792) 5386792..5387034 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN54_RS26405 (5387239) 5387239..5388156 + 918 WP_032418237.1 alpha/beta hydrolase -
LNN54_RS26410 (5388170) 5388170..5389111 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN54_RS26415 (5389156) 5389156..5389593 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN54_RS26420 (5389590) 5389590..5390450 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN54_RS26425 (5390444) 5390444..5391043 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223309 WP_002882817.1 NZ_CP086431:c5386792-5386409 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223309 NZ_CP118638:c5450139-5449861 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223309 WP_004150355.1 NZ_CP086431:c5387034-5386792 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223309 NZ_CP118638:c5449849-5449544 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References