Detailed information of TA system TA223308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5068440..5070121
• Replicon: chromosome
• Accession: NZ_CP086431
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-ESBL

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LNN54_RS24920
• Protein ID: WP_004146663.1
• Coordinates: 5068440..5069357 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LNN54_RS24925
• Protein ID: WP_032418188.1
• Coordinates: 5069363..5070121 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN54_RS24900 (5063746)	5063746..5065266	+	1521	WP_032418186.1	sugar ABC transporter ATP-binding protein	-
LNN54_RS24905 (5065263)	5065263..5066261	+	999	WP_002885172.1	ribose ABC transporter permease	-
LNN54_RS24910 (5066292)	5066292..5067233	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LNN54_RS24915 (5067308)	5067308..5068315	+	1008	WP_032418187.1	carbohydrate kinase family protein	-
LNN54_RS24920 (5068440)	5068440..5069357	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LNN54_RS24925 (5069363)	5069363..5070121	-	759	WP_032418188.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LNN54_RS24930 (5070207)	5070207..5070935	-	729	WP_004210017.1	response regulator transcription factor	-
LNN54_RS24935 (5071208)	5071208..5073355	+	2148	WP_077598926.1	formate dehydrogenase subunit alpha	-
LNN54_RS24940 (5073352)	5073352..5073495	-	144	WP_022631365.1	hypothetical protein	-
LNN54_RS24945 (5073701)	5073701..5074387	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T223308 WP_004146663.1 NZ_CP086431:c5069357-5068440 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T223308 NZ_CP118638:c4926480-4926133 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28620.99 Da
• Isoelectric Point: 10.0001

>AT223308 WP_032418188.1 NZ_CP086431:c5070121-5069363 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPETLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT223308 NZ_CP118638:c4926741-4926490 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: J2XCI1