Detailed information of TA system TA223295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1841564..1842154
• Replicon: chromosome
• Accession: NZ_CP086431
• Organism: Klebsiella pneumoniae strain AE-2247421-T5-ESBL

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LNN54_RS09015
• Protein ID: WP_023341911.1
• Coordinates: 1841822..1842154 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: LNN54_RS09010
• Protein ID: WP_000288812.1
• Coordinates: 1841564..1841821 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN54_RS08985 (1837474)	1837474..1837548	+	75	Protein_1766	type II toxin-antitoxin system PemK/MazF family toxin	-
LNN54_RS08990 (1837899)	1837899..1839647	+	1749	WP_032418698.1	hypothetical protein	-
LNN54_RS08995 (1839701)	1839701..1840282	+	582	WP_099184326.1	hypothetical protein	-
LNN54_RS09000 (1840565)	1840565..1841026	+	462	WP_004213450.1	hypothetical protein	-
LNN54_RS09005 (1841023)	1841023..1841229	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
LNN54_RS09010 (1841564)	1841564..1841821	+	258	WP_000288812.1	antitoxin	Antitoxin
LNN54_RS09015 (1841822)	1841822..1842154	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LNN54_RS09025 (1842476)	1842476..1843912	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LNN54_RS09035 (1844278)	1844278..1845732	-	1455	WP_004148975.1	AMP nucleosidase	-
LNN54_RS09040 (1845862)	1845862..1846106	-	245	Protein_1775	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T223295 WP_023341911.1 NZ_CP086431:1841822-1842154 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T223295 NZ_CP118633:2470611-2470895 [Salmonella enterica subsp. enterica serovar Anatum]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAGCTGGGACATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGCGTTGGAAAG
CGTGAAAATGATGCCATCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT223295 WP_000288812.1 NZ_CP086431:1841564-1841821 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT223295 NZ_CP118633:2470373-2470621 [Salmonella enterica subsp. enterica serovar Anatum]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACTGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0