Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5387184..5387809 Replicon chromosome
Accession NZ_CP086423
Organism Klebsiella pneumoniae strain AE-2247421-T20-MAC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN44_RS26400 Protein ID WP_002882817.1
Coordinates 5387184..5387567 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN44_RS26405 Protein ID WP_004150355.1
Coordinates 5387567..5387809 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN44_RS26385 (5384550) 5384550..5385452 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN44_RS26390 (5385449) 5385449..5386084 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN44_RS26395 (5386081) 5386081..5387010 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN44_RS26400 (5387184) 5387184..5387567 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN44_RS26405 (5387567) 5387567..5387809 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN44_RS26410 (5388014) 5388014..5388931 + 918 WP_032418237.1 alpha/beta hydrolase -
LNN44_RS26415 (5388945) 5388945..5389886 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN44_RS26420 (5389931) 5389931..5390368 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN44_RS26425 (5390365) 5390365..5391225 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN44_RS26430 (5391219) 5391219..5391818 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223289 WP_002882817.1 NZ_CP086423:c5387567-5387184 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223289 NZ_CP118633:348429-348797 [Salmonella enterica subsp. enterica serovar Anatum]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223289 WP_004150355.1 NZ_CP086423:c5387809-5387567 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223289 NZ_CP118633:348211-348432 [Salmonella enterica subsp. enterica serovar Anatum]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References