Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4788301..4789111 Replicon chromosome
Accession NZ_CP086423
Organism Klebsiella pneumoniae strain AE-2247421-T20-MAC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LNN44_RS23575 Protein ID WP_004178461.1
Coordinates 4788301..4788834 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LNN44_RS23580 Protein ID WP_002887278.1
Coordinates 4788845..4789111 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN44_RS23570 (4787132) 4787132..4788253 + 1122 WP_004214138.1 cupin domain-containing protein -
LNN44_RS23575 (4788301) 4788301..4788834 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LNN44_RS23580 (4788845) 4788845..4789111 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LNN44_RS23585 (4789214) 4789214..4790647 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
LNN44_RS23590 (4790637) 4790637..4791320 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LNN44_RS23595 (4791492) 4791492..4792877 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
LNN44_RS23600 (4792895) 4792895..4793239 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T223287 WP_004178461.1 NZ_CP086423:c4788834-4788301 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T223287 NZ_CP118631:4730882-4731157 [Mucilaginibacter sp. SJ]
ATGATTACTGAGTTTACACAGCATGGCTGGAACGATTTTGAGTACTGGTTGGAGAATGACCTCGAGGTTGTTGAAAAAAT
CAGGGAGCTAATTAAGTCAATTAAGCAGACTCCGTTTAAGGGTTTAGGAAAACCCGAACCATTAAGACATAGTTTAAAGG
GGTATTGGTCAAGAAGGATAACGGGTGAACACAGGCTTGTTTACCAGGTATCGGGCACAAAAGGTGATGATCAGAGGTGC
TCAATCGTACAATGCCGGTTTCATTATGGTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT223287 WP_002887278.1 NZ_CP086423:c4789111-4788845 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT223287 NZ_CP118631:4730615-4730878 [Mucilaginibacter sp. SJ]
ATGAAAGTACTAACAATTTCTTCTTTACGAAGTAAAATGAAATCTTATTTTGATGTGGTTAGTAATAATCAGGATGTTAT
TGTTGTACCAAGGAATAATAACGACGAGGATGCAGTTGTAATTATGTCTATAAAAGAGTATAACTCATTAACTGAAACCG
GGCACTTGCTATCAACCGCCGCTAATTCCAGGCGACTCGAAGAATCAATAGATCAATTGAAATCAGGAAAGACCATCACC
TATGATTTTGATTCCGGTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References