Detailed information of TA system TA022328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 81709..82290
• Replicon: plasmid plasmid_153kb
• Accession: NC_009705
• Organism: Yersinia pseudotuberculosis IP 31758

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: YPSIP31758_RS00760
• Protein ID: WP_041175657.1
• Coordinates: 81709..82041 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0U1QTL2
• Locus tag: YPSIP31758_RS00765
• Protein ID: WP_011988565.1
• Coordinates: 82045..82290 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YPSIP31758_RS00740	78329..79402	-	1074	WP_011988612.1	DUF4935 domain-containing protein	-
YPSIP31758_RS00745	79827..80105	-	279	WP_041175654.1	hypothetical protein	-
YPSIP31758_RS00750	80522..80860	+	339	WP_041175655.1	hypothetical protein	-
YPSIP31758_RS00755	81046..81588	-	543	WP_041175656.1	hypothetical protein	-
YPSIP31758_RS00760	81709..82041	-	333	WP_041175657.1	endoribonuclease MazF	Toxin
YPSIP31758_RS00765	82045..82290	-	246	WP_011988565.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
YPSIP31758_RS00770	82551..83390	-	840	WP_011988487.1	hypothetical protein	-
YPSIP31758_RS00775	83794..84912	-	1119	WP_011988604.1	hypothetical protein	-
YPSIP31758_RS00785	85320..85781	+	462	WP_041175659.1	hypothetical protein	-
YPSIP31758_RS24200	85756..85902	+	147	WP_165285902.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..153140	153140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-104)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12120.19 Da
• Isoelectric Point: 9.8470

>T22328 WP_041175657.1 NC_009705:c82041-81709 [Yersinia pseudotuberculosis IP 31758]
MIKNYVPEAGDIVWLDFDPQAGHEQAGHRPALVLSPAIYNEKVGLMLCCPMTTQIKGYPFEVIIAGTKPSAVLSDQIKSL
DWRKRKATRKGRVSALELSEVRAKSKALIG

Nucleotide

• Length: 333 bp

>T22328 NC_009705:c82041-81709 [Yersinia pseudotuberculosis IP 31758]
ATGATTAAAAATTACGTTCCTGAAGCTGGCGACATAGTGTGGCTTGATTTTGATCCACAGGCTGGCCATGAGCAAGCGGG
GCATAGACCTGCTCTTGTACTAAGCCCAGCGATATATAATGAAAAAGTTGGGTTAATGTTGTGTTGTCCGATGACGACGC
AGATTAAGGGCTATCCATTTGAAGTAATTATCGCAGGAACAAAGCCAAGTGCAGTTTTATCAGATCAAATTAAAAGCCTA
GACTGGCGTAAACGTAAAGCTACACGAAAAGGGCGTGTATCCGCGCTTGAATTATCTGAAGTAAGAGCTAAATCGAAGGC
TTTAATTGGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8847.34 Da
• Isoelectric Point: 5.8065

>AT22328 WP_011988565.1 NC_009705:c82290-82045 [Yersinia pseudotuberculosis IP 31758]
MAQVIVKKWGNSPSVRLPVAVMEAASLKIDDTVDIVVEDGRIVIVPVRAKEISLQSLLGRLTDDNIHREVDFGKPVGKEA
L

Nucleotide

• Length: 246 bp

>AT22328 NC_009705:c82290-82045 [Yersinia pseudotuberculosis IP 31758]
ATGGCACAAGTTATAGTAAAAAAATGGGGCAATAGTCCTTCTGTAAGACTGCCTGTTGCAGTTATGGAAGCCGCCAGTTT
GAAGATAGATGATACTGTAGATATCGTGGTTGAAGATGGGCGTATTGTTATTGTGCCAGTACGGGCTAAAGAAATATCGC
TGCAATCGCTATTAGGTAGACTAACTGACGATAATATCCACCGCGAAGTAGATTTCGGGAAGCCTGTAGGGAAAGAGGCA
CTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0U1QTL2