Detailed information of TA system TA223273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 342974..343560
• Replicon: chromosome
• Accession: NZ_CP086423
• Organism: Klebsiella pneumoniae strain AE-2247421-T20-MAC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LNN44_RS01625
• Protein ID: WP_002920800.1
• Coordinates: 343192..343560 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LNN44_RS01620
• Protein ID: WP_004174006.1
• Coordinates: 342974..343195 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN44_RS01600 (339131)	339131..340057	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LNN44_RS01605 (340054)	340054..341331	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LNN44_RS01610 (341328)	341328..342095	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LNN44_RS01615 (342097)	342097..342810	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LNN44_RS01620 (342974)	342974..343195	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LNN44_RS01625 (343192)	343192..343560	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LNN44_RS01630 (343833)	343833..345149	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LNN44_RS01635 (345256)	345256..346143	+	888	WP_032418302.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LNN44_RS01640 (346140)	346140..346985	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LNN44_RS01645 (346987)	346987..348057	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	337960..351582	13622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T223273 WP_002920800.1 NZ_CP086423:343192-343560 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T223273 NZ_CP118629:c6898-6545 [Pantoea dispersa]
ATGTGGGAAATCATTTTTACCGACACCTTCCGGGAATGGTTGCGGGCACAGGACAACAAACTGAAAAAACGCATCGCTGC
GGCCCTGTTAAACCTGCAGCATTATGGCCCGTTGCTGCCACGGCCCTATGCCGATACGGTTAACGGATCACGCTTCCACA
ACATGAAAGAACTGAGAATACAGTACGCTGGTCAGCCAATTCGGGCGCTATACGCATTCGACACGCAACGCAAAGCGGTC
ATTTTGTGTGCTGGCAATAAATCCGGAGACAAACAATTTTATGCGCGGATGATATGCCTTGCAGATCAATTGTTCACGGC
TTACCTCAATAACGCAGAGGAAAATCATTCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT223273 WP_004174006.1 NZ_CP086423:342974-343195 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT223273 NZ_CP118629:c6548-6243 [Pantoea dispersa]
ATGAACGACTTACAAAAATTTATCGAGAGTGAGTTTACTGAAGCTGAGCAAACTGACATTCAGCTGATGGCAGATGAGCT
GATTCTCGAAACCGGCCTGCAACTGCTGCGGGAAGAGTTAGCGTTATCGCAGAGAACCGTTGCGGAAGCGATGGGCGTTA
AGCAGCCGCGTATCACGCAAATTGAGCAGGGCGGCGCCGATCAGCGTTTGATCACCATTAAGCGCTACATTGAAGCGATG
GGCGGCAAGCTAAGCCTGCTGATTGAAATGCCGGACGGCTCGGGTGGAAGAATTCTGCGCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7