Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5386408..5387033 Replicon chromosome
Accession NZ_CP086416
Organism Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN42_RS26390 Protein ID WP_002882817.1
Coordinates 5386408..5386791 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN42_RS26395 Protein ID WP_004150355.1
Coordinates 5386791..5387033 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN42_RS26375 (5383774) 5383774..5384676 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN42_RS26380 (5384673) 5384673..5385308 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN42_RS26385 (5385305) 5385305..5386234 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN42_RS26390 (5386408) 5386408..5386791 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN42_RS26395 (5386791) 5386791..5387033 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN42_RS26400 (5387238) 5387238..5388155 + 918 WP_032418237.1 alpha/beta hydrolase -
LNN42_RS26405 (5388169) 5388169..5389110 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN42_RS26410 (5389155) 5389155..5389592 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN42_RS26415 (5389589) 5389589..5390449 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN42_RS26420 (5390443) 5390443..5391042 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223269 WP_002882817.1 NZ_CP086416:c5386791-5386408 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223269 NZ_CP118614:5655678-5655929 [Nocardiopsis sp. DZFXJ 01]
GTGAAGACGATGAGCTACTCGGAGTCCCGTGCCAACTACGCGGCCACGCTCGACTCCGTCGTCGACGACCGCGAGGAGGT
GGTGATCACCAGAGCCGGGCACGAGCCCGTGGTCATGATGGCGCTGTCGGAGTACGAGACCCTCAAGGAGACCGCCTACC
TCCTCCGCAGCCCGGAGAACGTGCGGCGCCTGGTCACCGCCATCGAGGAGCTCGAGTCCGACCAGGGCATAGAACGGGAC
CTGGCCGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223269 WP_004150355.1 NZ_CP086416:c5387033-5386791 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223269 NZ_CP118614:5655926-5656186 [Nocardiopsis sp. DZFXJ 01]
ATGAGACTCGTCTGGGCACAGAGCGCGTGGGAGGACTACGTCTGGTGGCAGGGACAGGACCACAGGATCCTCAAACGCAT
CAATGCCCTGATCAAGGACATCGACCGCAACGGAAACGACGGTATGGGCAAACCCGAGCCGCTGCGGCACGGTTTCCAGG
GCTACTGGTCCCGTCGCATCACCGACGAACACCGGCTGGTGTACAAGATCAGCGGGGATGAGATCCGGATAGCCGCCTGC
CGCTACCACTACGGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References