Detailed information of TA system TA223268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5068439..5070120
• Replicon: chromosome
• Accession: NZ_CP086416
• Organism: Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LNN42_RS24915
• Protein ID: WP_004146663.1
• Coordinates: 5068439..5069356 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LNN42_RS24920
• Protein ID: WP_032418188.1
• Coordinates: 5069362..5070120 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN42_RS24895 (5063745)	5063745..5065265	+	1521	WP_032418186.1	sugar ABC transporter ATP-binding protein	-
LNN42_RS24900 (5065262)	5065262..5066260	+	999	WP_002885172.1	ribose ABC transporter permease	-
LNN42_RS24905 (5066291)	5066291..5067232	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LNN42_RS24910 (5067307)	5067307..5068314	+	1008	WP_032418187.1	carbohydrate kinase family protein	-
LNN42_RS24915 (5068439)	5068439..5069356	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LNN42_RS24920 (5069362)	5069362..5070120	-	759	WP_032418188.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LNN42_RS24925 (5070206)	5070206..5070934	-	729	WP_004210017.1	response regulator transcription factor	-
LNN42_RS24930 (5071207)	5071207..5073354	+	2148	WP_077598926.1	formate dehydrogenase subunit alpha	-
LNN42_RS24935 (5073351)	5073351..5073494	-	144	WP_022631365.1	hypothetical protein	-
LNN42_RS24940 (5073700)	5073700..5074386	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T223268 WP_004146663.1 NZ_CP086416:c5069356-5068439 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T223268 NZ_CP118614:1743783-1744055 [Nocardiopsis sp. DZFXJ 01]
ATGACGTGGAAAGTTGAGCTCCACGACGATGCACGTCGTGACCTGCGAAAACTGGACCCACAGGTGGTACGGAGAATTCT
CCGGTTCCTGAAGGATCGGGTGGAGGGAGCGGACGACCCGCGTTGCCTCGGTGAGGCGTTGAAGGGCAGTCGCCTCGGTG
ACCTGTGGCGTTATCGTGTCGGCGACTACCGCGTGGTCGTCGACATCCGGGACGGACTCCTCGTCGTTCTCGTGGTGCAC
ATCGCCCATCGCAGTCAGGTGTACCGGGACTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28620.99 Da
• Isoelectric Point: 10.0001

>AT223268 WP_032418188.1 NZ_CP086416:c5070120-5069362 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPETLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT223268 NZ_CP118614:1743575-1743799 [Nocardiopsis sp. DZFXJ 01]
ATGCTGGCATTGAGACTTCCGGCGGAGATCGAGGACAGACTCGAAGCCCTCGCGAAGGAGACTGGCAGGTCGAAGAGCTT
CTACGCCCGGGAAGCGATCGTTGCTCACCTGGACGAGCTGGAGGAGGTCTATCTGGCCGAACGCAGGCTGGAGGACCTCA
GGGCGGGGCGCAGCGGTACGACACCGCTCTCAGAGGTAATGGACGAACATGACGTGGAAAGTTGA

Structures

Toxin

• AlphaFold DB: J2XCI1