Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4191144..4191763 Replicon chromosome
Accession NZ_CP086416
Organism Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LNN42_RS20680 Protein ID WP_002892050.1
Coordinates 4191545..4191763 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LNN42_RS20675 Protein ID WP_002892066.1
Coordinates 4191144..4191518 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN42_RS20665 (4186296) 4186296..4187489 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN42_RS20670 (4187512) 4187512..4190658 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LNN42_RS20675 (4191144) 4191144..4191518 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LNN42_RS20680 (4191545) 4191545..4191763 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LNN42_RS20685 (4191922) 4191922..4192488 + 567 WP_002892030.1 maltose O-acetyltransferase -
LNN42_RS20690 (4192460) 4192460..4192600 - 141 WP_004147370.1 hypothetical protein -
LNN42_RS20695 (4192621) 4192621..4193091 + 471 WP_002892026.1 YlaC family protein -
LNN42_RS20700 (4193066) 4193066..4194517 - 1452 WP_032419337.1 PLP-dependent aminotransferase family protein -
LNN42_RS20705 (4194618) 4194618..4195316 + 699 WP_002892021.1 GNAT family protein -
LNN42_RS20710 (4195313) 4195313..4195453 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LNN42_RS20715 (4195453) 4195453..4195716 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223266 WP_002892050.1 NZ_CP086416:4191545-4191763 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T223266 NZ_CP118614:967433-967690 [Nocardiopsis sp. DZFXJ 01]
GTGAGGCGACTGGTCTTCGAGGACCACGGCTGGGAGGGCTGCACATCCTGGCTCAAGACCGACCGCAAGGTACTCGCCCG
GATCAACAAGCTGATCGGGGAAATCCGCCGGGATCCCTTCACCGGCATAGGCGAACCGGAGCCCCTGAAGTACCACCTGC
CCGGCGCCTGGTCGCGGCGCATCGACGACGAGCACCGCCCGGTCCACGTCGTCACGGAGGACGAGATCGTCGTCATCGCC
GCTCGCTACCACTACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT223266 WP_002892066.1 NZ_CP086416:4191144-4191518 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT223266 NZ_CP118614:967167-967436 [Nocardiopsis sp. DZFXJ 01]
ATGTCCATCACCGCCAGCGAAGCGCGCAGGTCCCTCTTCCCGTTGATCAAGAAGGTCAACGACGACCACGACGCCATCGA
GATCGTGTCCAGGCACGGCAACGCGGTGCTCGTCTCCGCCGAGGACTACGCCGCGCTGCGTGAGGGCGCCTACCTCCTGC
GTTCGCCCGCGAACGCCCGGCGCCTGCTCAAGGCCTACGAGAACGCTCTGGACGGCACCGGTGTCTCCGAGCGCGGGTTG
ATCGGTCCCGACGTCGAGGACGCGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References