Detailed information of TA system TA223264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2796706..2798294
• Replicon: chromosome
• Accession: NZ_CP086416
• Organism: Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LNN42_RS13875
• Protein ID: WP_004184027.1
• Coordinates: 2796706..2798028 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LNN42_RS13880
• Protein ID: WP_004176198.1
• Coordinates: 2798028..2798294 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN42_RS13860 (2791762)	2791762..2794263	+	2502	WP_032418977.1	fimbria/pilus outer membrane usher protein	-
LNN42_RS13865 (2794251)	2794251..2795255	+	1005	WP_016946380.1	fimbrial protein	-
LNN42_RS13870 (2795397)	2795397..2796689	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LNN42_RS13875 (2796706)	2796706..2798028	-	1323	WP_004184027.1	type II toxin-antitoxin system HipA family toxin	Toxin
LNN42_RS13880 (2798028)	2798028..2798294	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LNN42_RS13885 (2798560)	2798560..2798886	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
LNN42_RS13890 (2798883)	2798883..2799383	-	501	WP_032418978.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LNN42_RS13895 (2799361)	2799361..2800740	-	1380	WP_032418979.1	nucleobase:cation symporter-2 family protein	-
LNN42_RS13900 (2801086)	2801086..2802240	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LNN42_RS13905 (2802221)	2802221..2803144	-	924	WP_032418980.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T223264 WP_004184027.1 NZ_CP086416:c2798028-2796706 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T223264 NZ_CP118614:375103-375504 [Nocardiopsis sp. DZFXJ 01]
ATGGCCGTTCTTCTGGACGCCAACGTCCTCATCGCCCTGGTGGTGGACGACCACGTGCACCACCGGGCCGCGGAGGCCTG
GATCACCGAGGTCGACACCGCCTTCGCGACCTGCCCGATCACCCAGGGAAGCCTGGTGCGCCTCCTCCTCCGGGAAGGGC
AGGACGCGGAGATCGCCAGGAGGGTCGTCGCCGCTTTGGCGGCCGACGAGCGGCACGAGTTCTGGCCCGACTCCATCGCG
TACACGGACGTCATCATGCACGGTGTGATCGGTCACCGGCAGGTGACCGACGCTTACCTCGCGCAACTCGCCAGGGTGCA
CGACGGGCGACTCGCCACGTTCGACCAGGGACTCGCCAAGCTCCACGGTGACGTGGCCGAACTGGTGCCCACCCCCGGGT
GA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT223264 WP_004176198.1 NZ_CP086416:c2798294-2798028 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT223264 NZ_CP118614:374873-375103 [Nocardiopsis sp. DZFXJ 01]
ATGCGAACGACGATCGACATTCCAGCGGACCTGCACCAGCAGGCCGTTTCGATAGCCCGGGACACGTCGAGGAGCCTGAG
CGAGACGGTCACCGATCTGATGCGTCGGGGACTGGGGCAGAGCGGCCCGGTCCGCGAGGTCTCGCAGAGCCCCAGGACCG
GGCTGCCCTTGGTCCACCTGGGTTCCGTCATCACCACCGAGGACGTGCGCGCACTGGAGGACGAGGACTGA