Detailed information of TA system TA223255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1841566..1842156
• Replicon: chromosome
• Accession: NZ_CP086416
• Organism: Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LNN42_RS09015
• Protein ID: WP_023341911.1
• Coordinates: 1841824..1842156 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: LNN42_RS09010
• Protein ID: WP_000288812.1
• Coordinates: 1841566..1841823 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN42_RS08985 (1837476)	1837476..1837550	+	75	Protein_1766	type II toxin-antitoxin system PemK/MazF family toxin	-
LNN42_RS08990 (1837901)	1837901..1839649	+	1749	WP_032418698.1	hypothetical protein	-
LNN42_RS08995 (1839703)	1839703..1840284	+	582	WP_099184326.1	hypothetical protein	-
LNN42_RS09000 (1840567)	1840567..1841028	+	462	WP_004213450.1	hypothetical protein	-
LNN42_RS09005 (1841025)	1841025..1841231	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
LNN42_RS09010 (1841566)	1841566..1841823	+	258	WP_000288812.1	antitoxin	Antitoxin
LNN42_RS09015 (1841824)	1841824..1842156	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LNN42_RS09025 (1842478)	1842478..1843914	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LNN42_RS09035 (1844280)	1844280..1845734	-	1455	WP_004148975.1	AMP nucleosidase	-
LNN42_RS09040 (1845864)	1845864..1846108	-	245	Protein_1775	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T223255 WP_023341911.1 NZ_CP086416:1841824-1842156 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T223255 NZ_CP118607:c3927484-3927188 [Pseudomonas sp. PSKL.D1]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTGAAAGCCTTGGCTGCCGAAGGGCGCGTTGGCGTTACGGGTAC
TGCATTGAGAGGCGCCAGGACGCTGGGAATGGATTTTCCCGGCATCCTTGAAGTGGTTGCCCGCCTGGAACGCACTGATT
TCTTCAAGAGCATGACCAGCCATATCGATCACCGGGTTTGGCAGGACGTGTATCGCCGGCTTACGGCAACCGGGTACGTT
TATCTGAAAGTTTCCGTGATAGACGAGGTACTCATCGTGTCTTTCAAGGAGTTGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT223255 WP_000288812.1 NZ_CP086416:1841566-1841823 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT223255 NZ_CP118607:c3927185-3926784 [Pseudomonas sp. PSKL.D1]
ATGAGATGTCCAGCATGCGGCGGTGCGGAGCTTGCACCTGATGTTCAGGGTATGCCCTATAGTTACAAAGGCGTGGCGAC
CGTAATCACAGGCGTTAGTGGCGATTACTGTTCCGCCTGTGGAGAATGTGTTCTTGGCCACGATGAAGCCATGCGTGTCA
GTACTCTGATGACGGCGTTCGAGCGCCAAGTGAACGCAACGGTTGTGGACCCTTCGTTCATTGTTTCCATACGCCGGAAA
TTCGACCTCGACCAGCGTGAGGCGGGGGAGATCTTTGGTGGTGGGGTCAATGCATTCTCTCGCTATGAAAACGGCAAGAC
CACACCCCCAGTGGCGCTGATCAAGTTGTTCAAACTGCTCGATCGCCACCCGGAATTGTTCGAGGAAGTGCGTACCGCCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0