Detailed information of TA system TA223253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 342973..343559
• Replicon: chromosome
• Accession: NZ_CP086416
• Organism: Klebsiella pneumoniae strain AE-2247421-T20-ESBL

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LNN42_RS01625
• Protein ID: WP_002920800.1
• Coordinates: 343191..343559 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LNN42_RS01620
• Protein ID: WP_004174006.1
• Coordinates: 342973..343194 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN42_RS01600 (339130)	339130..340056	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LNN42_RS01605 (340053)	340053..341330	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LNN42_RS01610 (341327)	341327..342094	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LNN42_RS01615 (342096)	342096..342809	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LNN42_RS01620 (342973)	342973..343194	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LNN42_RS01625 (343191)	343191..343559	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LNN42_RS01630 (343832)	343832..345148	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LNN42_RS01635 (345255)	345255..346142	+	888	WP_032418302.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LNN42_RS01640 (346139)	346139..346984	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LNN42_RS01645 (346986)	346986..348056	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	340053..351581	11528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T223253 WP_002920800.1 NZ_CP086416:343191-343559 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T223253 NZ_CP118607:c1589276-1588983 [Pseudomonas sp. PSKL.D1]
ATGACTGACACCTTTTTTGAGTTTGACCCGGCCGACTACCTCACTACACCCGAGGCCATCGCACTATTCATGACCGACGC
CCTGGAAACAGGAGACGCCAGCTACGTGGCCAAAGCTGTCGGTGTGGTGGCACGGGCCAAAGGTATGACGGAGTTGGCCA
AAGAAACCGGCTTGTCTCGTGAACAGCTTTATCGCTCGTTCAGCGAGCACGGCAACCCAACGCTCAAATCATTTTTGGCG
GTAATGAAAGCACTGGGTATAGACATGACCGCAACGCCTCATCTGGCACGTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT223253 WP_004174006.1 NZ_CP086416:342973-343194 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT223253 NZ_CP118607:c1589571-1589269 [Pseudomonas sp. PSKL.D1]
ATGAAAAAAATCGAATCCAGCAGCTTCAGGAAGTGGGTGATCGGACTCAAAGATGCCAAGGCAAGAGTCAGGATCATCTC
GCGCATCAACCGACTCATGGAAGGCATATCCGGCGATGTCTCGCCGGTTGGGCATGGCGTCAGTGAACTCAGAATCCACC
TCGGGCCTGGCTACCGTGTGTACTTTCACCAATCGGGCGATACCTTGGTGATATTGCTTTGCGGTGGTGACAAAGATAGC
CAGCAACGAGACATCAAGGCTGCCCACCAGATTCTGCGCGCGTGGAGGATGCAAAATGACTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7