223252

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 35415..36016 Replicon plasmid pZW5B
Accession NZ_CP086414
Organism Lactiplantibacillus plantarum strain ZW5

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag LMJ47_RS16035 Protein ID WP_001748110.1
Coordinates 35672..36016 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag LMJ47_RS16030 Protein ID WP_001748109.1
Coordinates 35415..35678 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ47_RS16015 (LMJ47_16010) 32251..32463 - 213 WP_015063436.1 hypothetical protein -
LMJ47_RS16020 (LMJ47_16015) 33379..34644 - 1266 WP_063730351.1 hypothetical protein -
LMJ47_RS16025 (LMJ47_16020) 34742..35329 - 588 WP_024526978.1 site-specific integrase -
LMJ47_RS16030 (LMJ47_16025) 35415..35678 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LMJ47_RS16035 (LMJ47_16030) 35672..36016 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMJ47_RS16040 (LMJ47_16035) 36137..36349 - 213 WP_063730353.1 DUF3923 family protein -
LMJ47_RS16045 (LMJ47_16040) 36588..37076 + 489 WP_046783592.1 DUF536 domain-containing protein -
LMJ47_RS16050 (LMJ47_16045) 37781..38158 + 378 WP_003646093.1 YxeA family protein -
LMJ47_RS16055 (LMJ47_16050) 38304..38765 - 462 WP_228977985.1 hypothetical protein -
LMJ47_RS16060 (LMJ47_16055) 39537..40139 - 603 WP_228977988.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40831 40831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T223252 WP_001748110.1 NZ_CP086414:35672-36016 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T223252 NZ_CP118607:c1158224-1157904 [Pseudomonas sp. PSKL.D1]
ATGCTTTTCATCGAAACACCGATCTTTTCCAGAAGCGTTAAAGAGCTACTTGACGACGAAGCCTACCGATTACTGCAGAT
CCAGTTGATGCTTTCACCGGATTTGGGTGACCTGATGGAAGGCACCGGAGGTTTGAGAAAAGTGCGCGTTGCTGCTAATG
GGCGGGGCAAGCGAGGTGGAGCCAGAGCCATCTATTACCATTTCGTCGCTTCATCTCAGATAGCCATGCTGTACATCTAT
CCGAAGAATGAACAATCTGACTTGTCTACCGAACAATGCAAAGCACTTAAGGGTGTAGTCGAGCACTGGAGGCGCACATG
A

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT223252 WP_001748109.1 NZ_CP086414:35415-35678 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT223252 NZ_CP118607:c1157907-1157620 [Pseudomonas sp. PSKL.D1]
ATGAGTAAATTTTTTGAAGAGCTGCTGGAAAGCGTTCAACAAATGGATGAAATCGTTCAAGGCAAGCGCCCTGCTTCACG
GGTGTTTGAGATCGACGCCCTTCAAGTCAAGGCCATTCGTCAATCTACGGGATTATCACAGGCCCGCTTCGCCGGGATGA
TCGATGTCCAGGTCGCCACGCTAAGAAACTGGGAGCAAGGACGAAGGGAACCTACTGGCCCGGCGAAAGCCCTCTTGCGG
GCGATACGAAACGATCCCGACCACGTCCTGAAAGCATTGGCTCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References