Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 15001..15602 Replicon plasmid pZW5B
Accession NZ_CP086414
Organism Lactiplantibacillus plantarum strain ZW5

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag LMJ47_RS15930 Protein ID WP_001748110.1
Coordinates 15258..15602 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag LMJ47_RS15925 Protein ID WP_001748109.1
Coordinates 15001..15264 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ47_RS15910 (LMJ47_15905) 11835..12047 - 213 WP_015063436.1 hypothetical protein -
LMJ47_RS15915 (LMJ47_15910) 12965..14230 - 1266 WP_063730351.1 hypothetical protein -
LMJ47_RS15920 (LMJ47_15915) 14328..14915 - 588 WP_024526978.1 site-specific integrase -
LMJ47_RS15925 (LMJ47_15920) 15001..15264 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LMJ47_RS15930 (LMJ47_15925) 15258..15602 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMJ47_RS15935 (LMJ47_15930) 15723..15935 - 213 WP_063730353.1 DUF3923 family protein -
LMJ47_RS15940 (LMJ47_15935) 16174..16662 + 489 WP_046783592.1 DUF536 domain-containing protein -
LMJ47_RS15945 (LMJ47_15940) 17367..17744 + 378 WP_003646093.1 YxeA family protein -
LMJ47_RS15950 (LMJ47_15945) 17890..18351 - 462 WP_228977985.1 hypothetical protein -
LMJ47_RS15955 (LMJ47_15950) 19123..19725 - 603 WP_228977988.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40831 40831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T223251 WP_001748110.1 NZ_CP086414:15258-15602 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T223251 NZ_CP118607:867487-867714 [Pseudomonas sp. PSKL.D1]
ATGCGCAGTCGAGATGTGATTCAGATTATTGAGGCGGATGGTTGGTACTTGGTAGATGTGAAAGGGAGCCATCACCAGTT
TAAGCACCCCGCGAAAAAGGGTCGGGTTACGGTTCCTCATCCCAAGACAGACCTGCCTAAAGGCACAGTTCACAACATTT
TGAAGCAGGCAGGTCTGAAGCAGACCCGTTGCGTAAACAGCCCAAATATCACTGGAGGCTGCCAATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT223251 WP_001748109.1 NZ_CP086414:15001-15264 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT223251 NZ_CP118607:867711-868118 [Pseudomonas sp. PSKL.D1]
ATGAAATTCCCTGTCGTACTACACAAAGACCACGGCTCGGACTACGGGGTAACAGTACCCGACGTGCCCGGCTGTTTCTC
GGCTGGCTGTAATGTCGCTGAGGCTTTGGACAATGTTCAGGAGGCACTGTCGCTGCATTTTGAAGGTTTGGTGGCGGACA
ATGAGCCACTGCCGCAAGCTCAGGAGATCGACGTGCATGTGGTCAACCCCGATTACGTTGGCGGTGTCTGGGCCGTTGTG
GATTTCGATGTGACGCCCTACCTGGGAAAAGCGGTGCGATTCAATGCCACATTGCCTGAGAACTTGCTTCAGCGAATCGA
TGAAAAAGTGAAGCGTGATCATCGTTACGCGTCGAGGTCTGGCTTTCTCGCCTCTGCGGCGCTGCGAGAGTTGGCACTGG
CGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References