Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 251311..251953 Replicon chromosome
Accession NC_009674
Organism Bacillus cytotoxicus NVH 391-98

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCER98_RS01375 Protein ID WP_000635963.1
Coordinates 251603..251953 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag BCER98_RS01370 Protein ID WP_000004571.1
Coordinates 251311..251598 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCER98_RS01350 247691..248251 - 561 WP_011983362.1 rhomboid family intramembrane serine protease -
BCER98_RS01355 248342..248701 + 360 WP_011983363.1 holo-ACP synthase -
BCER98_RS01360 248857..249807 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
BCER98_RS01365 249928..251097 + 1170 WP_011983365.1 alanine racemase -
BCER98_RS01370 251311..251598 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
BCER98_RS01375 251603..251953 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCER98_RS01380 252018..254186 + 2169 WP_011983366.1 RNA-binding transcriptional accessory protein -
BCER98_RS21570 254260..254394 - 135 WP_087097560.1 cortex morphogenetic protein CmpA -
BCER98_RS01385 254773..255237 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T22324 WP_000635963.1 NC_009674:251603-251953 [Bacillus cytotoxicus NVH 391-98]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T22324 NC_009674:251603-251953 [Bacillus cytotoxicus NVH 391-98]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCAGCGATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACGCATGTGGAGATTGATGCGAAGAAGTACGGATTTGAGAGGGATTCTGTGATCTTGCTCGAGCAGATT
CGAACAATTGATAAGCAACGCTTAACGGACAAAATCACACATTTAGATGAAGTGATGATGATTCGTGTAGATGAGGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT22324 WP_000004571.1 NC_009674:251311-251598 [Bacillus cytotoxicus NVH 391-98]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT22324 NC_009674:251311-251598 [Bacillus cytotoxicus NVH 391-98]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTGACGGAATTGGACGGGATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAAAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References