Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2881807..2882378 Replicon chromosome
Accession NZ_CP086411
Organism Enterococcus faecalis strain R5

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag LMJ46_RS14315 Protein ID WP_002360937.1
Coordinates 2881807..2882148 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag LMJ46_RS14320 Protein ID WP_002367500.1
Coordinates 2882148..2882378 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ46_RS14310 (2877822) 2877822..2881436 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
LMJ46_RS14315 (2881807) 2881807..2882148 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMJ46_RS14320 (2882148) 2882148..2882378 - 231 WP_002367500.1 hypothetical protein Antitoxin
LMJ46_RS14325 (2882433) 2882433..2882612 - 180 WP_111997057.1 hypothetical protein -
LMJ46_RS14330 (2882792) 2882792..2883007 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
LMJ46_RS14335 (2883146) 2883146..2884138 + 993 WP_002389493.1 biotin--[acetyl-CoA-carboxylase] ligase -
LMJ46_RS14340 (2884402) 2884402..2885040 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
LMJ46_RS14345 (2885726) 2885726..2887342 + 1617 WP_002389442.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T223235 WP_002360937.1 NZ_CP086411:c2882148-2881807 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T223235 NZ_CP118601:1898501-1898755 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT223235 WP_002367500.1 NZ_CP086411:c2882378-2882148 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT223235 NZ_CP118601:1898253-1898504 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References