Detailed information of TA system TA022322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 82156..82678
• Replicon: plasmid pOANT01
• Accession: NC_009669
• Organism: Ochrobactrum anthropi ATCC 49188

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A6X7M9
• Locus tag: OANT_RS23410
• Protein ID: WP_011982866.1
• Coordinates: 82156..82443 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A6X7N0
• Locus tag: OANT_RS23415
• Protein ID: WP_011982865.1
• Coordinates: 82433..82678 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OANT_RS23385	77279..77620	+	342	WP_011982871.1	DUF736 family protein	-
OANT_RS23390	77741..77941	+	201	WP_011982870.1	antitoxin VbhA family protein	-
OANT_RS23395	78457..79860	+	1404	WP_011982869.1	group II intron reverse transcriptase/maturase	-
OANT_RS23400	79936..80985	+	1050	WP_011982868.1	Fic family protein	-
OANT_RS23405	81199..82086	+	888	WP_011982867.1	DUF1738 domain-containing protein	-
OANT_RS23410	82156..82443	-	288	WP_011982866.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OANT_RS23415	82433..82678	-	246	WP_011982865.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OANT_RS23420	82839..83327	-	489	WP_049768461.1	helix-turn-helix transcriptional regulator	-
OANT_RS23425	83506..83883	-	378	WP_011982863.1	hypothetical protein	-
OANT_RS26220	83886..84056	-	171	WP_162024451.1	ribbon-helix-helix protein, CopG family	-
OANT_RS23435	84304..85017	-	714	WP_011982861.1	ParA family protein	-
OANT_RS23440	85496..85978	+	483	WP_011982860.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..170351	170351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10998.80 Da
• Isoelectric Point: 10.6834

>T22322 WP_011982866.1 NC_009669:c82443-82156 [Ochrobactrum anthropi ATCC 49188]
MPYKLEFLPSALKEWNKLGATIQQQLKKKLRERLETPRVVSASLHGMPDHYKIKLRQLGYRLVYSVNDDTVTVLVVAVGK
RERGDVYNTARSRTQ

Nucleotide

• Length: 288 bp

>T22322 NC_009669:c82443-82156 [Ochrobactrum anthropi ATCC 49188]
ATGCCTTATAAGCTTGAATTCTTGCCATCGGCTCTTAAAGAGTGGAATAAACTCGGCGCGACAATCCAGCAGCAATTGAA
GAAGAAACTACGCGAACGATTGGAAACGCCGCGCGTAGTGAGTGCTTCACTTCATGGTATGCCCGACCACTATAAGATAA
AACTACGTCAATTGGGCTATCGGCTGGTTTATTCCGTCAACGACGACACTGTGACGGTGTTGGTTGTGGCCGTTGGAAAA
CGTGAGCGTGGCGATGTTTATAATACCGCTCGCTCCAGAACACAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8886.16 Da
• Isoelectric Point: 4.2813

>AT22322 WP_011982865.1 NC_009669:c82678-82433 [Ochrobactrum anthropi ATCC 49188]
MQRVEANIAVSVSDLKKSPSAVMDEANGEAVAVLNHNRIMAYMVPAGVYEAMLEQLDDIRLTEIIRNRADEKGISVDIDA
L

Nucleotide

• Length: 246 bp

>AT22322 NC_009669:c82678-82433 [Ochrobactrum anthropi ATCC 49188]
ATGCAACGTGTGGAAGCAAATATAGCGGTTAGCGTTTCAGACCTAAAAAAAAGTCCGTCCGCCGTAATGGACGAGGCTAA
TGGCGAGGCTGTGGCCGTTCTTAATCATAATCGAATTATGGCTTATATGGTTCCCGCTGGGGTCTATGAAGCCATGCTCG
AACAGCTGGATGATATTCGCCTCACTGAGATAATCCGGAATCGAGCCGACGAAAAAGGAATTTCGGTCGATATCGATGCC
TTATAA

Structures

Toxin

• AlphaFold DB: A6X7M9

Antitoxin

• AlphaFold DB: A6X7N0