Detailed information of TA system TA223215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1127..1652
• Replicon: plasmid pPOL9B
• Accession: NZ_CP086410
• Organism: Enterobacter cloacae strain POL9

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: LMJ45_RS24165
• Protein ID: WP_016946795.1
• Coordinates: 1347..1652 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: LMJ45_RS24160
• Protein ID: WP_004197642.1
• Coordinates: 1127..1345 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ45_RS24160 (LMJ45_24160)	1127..1345	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LMJ45_RS24165 (LMJ45_24165)	1347..1652	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
LMJ45_RS24170 (LMJ45_24170)	1810..2319	+	510	WP_052522797.1	hypothetical protein	-
LMJ45_RS24175 (LMJ45_24175)	2498..3274	+	777	WP_049245386.1	site-specific integrase	-
LMJ45_RS24180 (LMJ45_24180)	3332..3589	-	258	WP_000764642.1	hypothetical protein	-
LMJ45_RS24185 (LMJ45_24185)	3718..3822	-	105	WP_032409716.1	hypothetical protein	-
LMJ45_RS24190 (LMJ45_24190)	4357..5223	+	867	WP_004118283.1	replication initiation protein	-
LMJ45_RS24195 (LMJ45_24195)	5508..5777	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..52962	52962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T223215 WP_016946795.1 NZ_CP086410:1347-1652 [Enterobacter cloacae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T223215 NZ_CP118586:2540334-2540612 [Vibrio harveyi]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAGAATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTATTGGGTCGAGGGCGATATCATCAGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT223215 WP_004197642.1 NZ_CP086410:1127-1345 [Enterobacter cloacae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT223215 NZ_CP118586:2540053-2540337 [Vibrio harveyi]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACGTCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA