Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1179184..1179804 Replicon chromosome
Accession NZ_CP086408
Organism Enterobacter cloacae strain POL9

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag LMJ45_RS05575 Protein ID WP_013095889.1
Coordinates 1179184..1179402 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LMJ45_RS05580 Protein ID WP_008499288.1
Coordinates 1179430..1179804 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ45_RS05545 (LMJ45_05545) 1175196..1175456 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
LMJ45_RS05550 (LMJ45_05550) 1175459..1175599 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LMJ45_RS05555 (LMJ45_05555) 1175596..1176306 - 711 WP_128310525.1 GNAT family protein -
LMJ45_RS05560 (LMJ45_05560) 1176408..1177868 + 1461 WP_229060010.1 PLP-dependent aminotransferase family protein -
LMJ45_RS05565 (LMJ45_05565) 1177840..1178307 - 468 WP_013095887.1 YlaC family protein -
LMJ45_RS05570 (LMJ45_05570) 1178423..1178974 - 552 WP_013095888.1 maltose O-acetyltransferase -
LMJ45_RS05575 (LMJ45_05575) 1179184..1179402 - 219 WP_013095889.1 HHA domain-containing protein Toxin
LMJ45_RS05580 (LMJ45_05580) 1179430..1179804 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LMJ45_RS05585 (LMJ45_05585) 1180317..1183463 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
LMJ45_RS05590 (LMJ45_05590) 1183486..1184679 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T223200 WP_013095889.1 NZ_CP086408:c1179402-1179184 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T223200 NZ_CP118567:c4686119-4685808 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT223200 WP_008499288.1 NZ_CP086408:c1179804-1179430 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT223200 NZ_CP118567:c4685807-4685517 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References