Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 635010..635586 Replicon chromosome
Accession NZ_CP086408
Organism Enterobacter cloacae strain POL9

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag LMJ45_RS03055 Protein ID WP_023620650.1
Coordinates 635010..635297 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5E1AP18
Locus tag LMJ45_RS03060 Protein ID WP_023620649.1
Coordinates 635284..635586 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ45_RS03030 (LMJ45_03030) 630073..630768 + 696 WP_045295461.1 winged helix-turn-helix domain-containing protein -
LMJ45_RS03035 (LMJ45_03035) 630972..631442 + 471 WP_013095432.1 MarR family transcriptional regulator -
LMJ45_RS03040 (LMJ45_03040) 631439..632506 + 1068 WP_128311826.1 HlyD family secretion protein -
LMJ45_RS03045 (LMJ45_03045) 632496..633572 + 1077 WP_128311828.1 DUF2955 domain-containing protein -
LMJ45_RS03050 (LMJ45_03050) 633569..634840 - 1272 WP_128311831.1 DUF445 domain-containing protein -
LMJ45_RS03055 (LMJ45_03055) 635010..635297 + 288 WP_023620650.1 BrnT family toxin Toxin
LMJ45_RS03060 (LMJ45_03060) 635284..635586 + 303 WP_023620649.1 BrnA antitoxin family protein Antitoxin
LMJ45_RS03065 (LMJ45_03065) 635617..636255 - 639 WP_128311833.1 LysE family translocator -
LMJ45_RS03070 (LMJ45_03070) 636304..637047 - 744 WP_128311835.1 AraC family transcriptional regulator -
LMJ45_RS03075 (LMJ45_03075) 637185..638555 + 1371 WP_128311837.1 NAD-dependent succinate-semialdehyde dehydrogenase -
LMJ45_RS03080 (LMJ45_03080) 638597..638917 - 321 WP_013095439.1 hypothetical protein -
LMJ45_RS03085 (LMJ45_03085) 638917..639462 - 546 WP_013095440.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T223199 WP_023620650.1 NZ_CP086408:635010-635297 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

>T223199 NZ_CP118567:c3896105-3895614 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11414.05 Da        Isoelectric Point: 10.3694

>AT223199 WP_023620649.1 NZ_CP086408:635284-635586 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT223199 NZ_CP118567:c3896395-3896102 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP18

References