Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 628378..629172 Replicon chromosome
Accession NZ_CP086408
Organism Enterobacter cloacae strain POL9

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag LMJ45_RS03010 Protein ID WP_013095427.1
Coordinates 628378..628899 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag LMJ45_RS03015 Protein ID WP_013095428.1
Coordinates 628903..629172 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ45_RS02985 (LMJ45_02985) 623705..623866 + 162 WP_013095422.1 DUF1127 domain-containing protein -
LMJ45_RS02990 (LMJ45_02990) 623953..625341 - 1389 WP_128311817.1 PLP-dependent aminotransferase family protein -
LMJ45_RS02995 (LMJ45_02995) 625444..625923 + 480 WP_128311819.1 carboxymuconolactone decarboxylase family protein -
LMJ45_RS03000 (LMJ45_03000) 625910..626812 - 903 WP_023620661.1 LysR family transcriptional regulator -
LMJ45_RS03005 (LMJ45_03005) 626913..628328 + 1416 WP_128311821.1 aldehyde dehydrogenase family protein -
LMJ45_RS03010 (LMJ45_03010) 628378..628899 - 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
LMJ45_RS03015 (LMJ45_03015) 628903..629172 - 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
LMJ45_RS03020 (LMJ45_03020) 629387..629710 + 324 WP_023620659.1 antibiotic biosynthesis monooxygenase -
LMJ45_RS03025 (LMJ45_03025) 629703..630101 + 399 WP_128311824.1 amino acid-binding protein -
LMJ45_RS03030 (LMJ45_03030) 630073..630768 + 696 WP_045295461.1 winged helix-turn-helix domain-containing protein -
LMJ45_RS03035 (LMJ45_03035) 630972..631442 + 471 WP_013095432.1 MarR family transcriptional regulator -
LMJ45_RS03040 (LMJ45_03040) 631439..632506 + 1068 WP_128311826.1 HlyD family secretion protein -
LMJ45_RS03045 (LMJ45_03045) 632496..633572 + 1077 WP_128311828.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T223198 WP_013095427.1 NZ_CP086408:c628899-628378 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T223198 NZ_CP118567:c3803086-3802676 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATTAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT223198 WP_013095428.1 NZ_CP086408:c629172-628903 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT223198 NZ_CP118567:c3803333-3803067 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References