Detailed information of TA system TA223197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 538177..538693
• Replicon: chromosome
• Accession: NZ_CP086408
• Organism: Enterobacter cloacae strain POL9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LMJ45_RS02610
• Protein ID: WP_128312323.1
• Coordinates: 538409..538693 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: LMJ45_RS02605
• Protein ID: WP_008501400.1
• Coordinates: 538177..538419 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ45_RS02590 (LMJ45_02590)	534205..534945	+	741	WP_094085364.1	KDGP aldolase family protein	-
LMJ45_RS02595 (LMJ45_02595)	535033..536169	+	1137	WP_128312318.1	lactonase family protein	-
LMJ45_RS02600 (LMJ45_02600)	536189..538099	+	1911	WP_128312321.1	PRD domain-containing protein	-
LMJ45_RS02605 (LMJ45_02605)	538177..538419	+	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LMJ45_RS02610 (LMJ45_02610)	538409..538693	+	285	WP_128312323.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMJ45_RS02615 (LMJ45_02615)	538697..539161	-	465	WP_128312325.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LMJ45_RS02620 (LMJ45_02620)	539284..541422	-	2139	WP_013095357.1	anaerobic ribonucleoside-triphosphate reductase	-
LMJ45_RS02625 (LMJ45_02625)	541796..543439	-	1644	WP_128312327.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10956.90 Da
• Isoelectric Point: 10.5826

>T223197 WP_128312323.1 NZ_CP086408:538409-538693 [Enterobacter cloacae]
MTYELAFDPRALKEWSKLGETVKKQFRKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVIAIGK
REKLTVYHDANKRL

Nucleotide

• Length: 285 bp

>T223197 NZ_CP118567:3521213-3521593 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT223197 WP_008501400.1 NZ_CP086408:538177-538419 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT223197 NZ_CP118567:3520854-3521180 [Enterobacter hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3