Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 283411..284007 Replicon chromosome
Accession NZ_CP086408
Organism Enterobacter cloacae strain POL9

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M7H115
Locus tag LMJ45_RS01335 Protein ID WP_029882644.1
Coordinates 283705..284007 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0F1AUP1
Locus tag LMJ45_RS01330 Protein ID WP_013095028.1
Coordinates 283411..283698 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ45_RS01325 (LMJ45_01325) 281783..283414 + 1632 WP_013095027.1 Na/Pi cotransporter family protein -
LMJ45_RS01330 (LMJ45_01330) 283411..283698 - 288 WP_013095028.1 putative addiction module antidote protein Antitoxin
LMJ45_RS01335 (LMJ45_01335) 283705..284007 - 303 WP_029882644.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMJ45_RS01340 (LMJ45_01340) 284205..285074 + 870 WP_023621411.1 23S rRNA pseudouridine(2604) synthase RluF -
LMJ45_RS01345 (LMJ45_01345) 285142..286086 - 945 WP_128311365.1 ketopantoate/pantoate/pantothenate transporter PanS -
LMJ45_RS01350 (LMJ45_01350) 286252..286707 - 456 WP_128311363.1 hypothetical protein -
LMJ45_RS01355 (LMJ45_01355) 286789..288363 - 1575 WP_128311362.1 phage tail protein -
LMJ45_RS01360 (LMJ45_01360) 288336..288998 - 663 WP_128311360.1 phage tail protein I -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11457.30 Da        Isoelectric Point: 9.7662

>T223196 WP_029882644.1 NZ_CP086408:c284007-283705 [Enterobacter cloacae]
MKEIVQTESFRRWEQNLKDRRARTIIASRLFRLANGLAGDIKPVGEGICELRIHFGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T223196 NZ_CP118567:c2655581-2655478 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10070.62 Da        Isoelectric Point: 5.7807

>AT223196 WP_013095028.1 NZ_CP086408:c283698-283411 [Enterobacter cloacae]
MHKLTPYDPAIALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPAGD

Download         Length: 288 bp

>AT223196 NZ_CP118567:2655473-2655674 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M7H115

Antitoxin

Source ID Structure
AlphaFold DB A0A0F1AUP1

References