Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 176253..176857 Replicon chromosome
Accession NZ_CP086408
Organism Enterobacter cloacae strain POL9

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag LMJ45_RS00860 Protein ID WP_071843252.1
Coordinates 176253..176438 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Z1F6W0
Locus tag LMJ45_RS00865 Protein ID WP_013094943.1
Coordinates 176453..176857 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ45_RS00845 (LMJ45_00845) 172684..174222 + 1539 WP_020686520.1 aldehyde dehydrogenase family protein -
LMJ45_RS00850 (LMJ45_00850) 174219..175184 - 966 WP_062676828.1 LysR family transcriptional regulator -
LMJ45_RS00855 (LMJ45_00855) 175302..176042 + 741 WP_229059967.1 MipA/OmpV family protein -
LMJ45_RS00860 (LMJ45_00860) 176253..176438 + 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
LMJ45_RS00865 (LMJ45_00865) 176453..176857 + 405 WP_013094943.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LMJ45_RS00870 (LMJ45_00870) 176911..178866 - 1956 WP_128310247.1 glycoside hydrolase family 127 protein -
LMJ45_RS00875 (LMJ45_00875) 178877..180277 - 1401 WP_020686524.1 MFS transporter -
LMJ45_RS00880 (LMJ45_00880) 180502..181320 + 819 WP_229059969.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T223195 WP_071843252.1 NZ_CP086408:176253-176438 [Enterobacter cloacae]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T223195 NZ_CP118567:c2227210-2226725 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14915.74 Da        Isoelectric Point: 4.3036

>AT223195 WP_013094943.1 NZ_CP086408:176453-176857 [Enterobacter cloacae]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVNEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT223195 NZ_CP118567:c2227464-2227198 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F6W0

References