Detailed information of TA system TA223193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4722319..4722935
• Replicon: chromosome
• Accession: NZ_CP086405
• Organism: Enterobacter roggenkampii strain POL1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMJ44_RS22490
• Protein ID: WP_084832869.1
• Coordinates: 4722319..4722690 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LMJ44_RS22495
• Protein ID: WP_015569912.1
• Coordinates: 4722693..4722935 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ44_RS22475 (LMJ44_22480)	4719819..4720721	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
LMJ44_RS22480 (LMJ44_22485)	4720718..4721353	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
LMJ44_RS22485 (LMJ44_22490)	4721350..4722279	+	930	WP_008501833.1	formate dehydrogenase accessory protein FdhE	-
LMJ44_RS22490 (LMJ44_22495)	4722319..4722690	-	372	WP_084832869.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMJ44_RS22495 (LMJ44_22500)	4722693..4722935	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LMJ44_RS22500 (LMJ44_22505)	4723134..4724054	+	921	WP_063418381.1	alpha/beta hydrolase	-
LMJ44_RS22505 (LMJ44_22510)	4724063..4725004	-	942	WP_008501835.1	fatty acid biosynthesis protein FabY	-
LMJ44_RS22510 (LMJ44_22515)	4725049..4725486	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
LMJ44_RS22515 (LMJ44_22520)	4725483..4726364	-	882	WP_023326335.1	virulence factor BrkB family protein	-
LMJ44_RS22520 (LMJ44_22525)	4726358..4726957	-	600	WP_063418382.1	glucose-1-phosphatase	-
LMJ44_RS22525 (LMJ44_22530)	4727046..4727549	-	504	WP_229020397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13700.81 Da
• Isoelectric Point: 6.3290

>T223193 WP_084832869.1 NZ_CP086405:c4722690-4722319 [Enterobacter roggenkampii]
MEHMAVFDTNILIDLFNNRVEAADAIENTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T223193 NZ_CP118567:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT223193 WP_015569912.1 NZ_CP086405:c4722935-4722693 [Enterobacter roggenkampii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT223193 NZ_CP118567:30794-31210 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTTGCG
CCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGCTATTTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3