Detailed information of TA system TA223182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 1677452..1678249
• Replicon: chromosome
• Accession: NZ_CP086405
• Organism: Enterobacter roggenkampii strain POL1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LMJ44_RS07835
• Protein ID: WP_063417750.1
• Coordinates: 1677728..1678249 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: LMJ44_RS07830
• Protein ID: WP_008499829.1
• Coordinates: 1677452..1677721 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ44_RS07800 (LMJ44_07805)	1673075..1673518	-	444	WP_008499834.1	helix-turn-helix domain-containing protein	-
LMJ44_RS07805 (LMJ44_07810)	1673671..1674912	+	1242	WP_063417751.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
LMJ44_RS07810 (LMJ44_07815)	1674948..1675175	-	228	WP_008499832.1	YccJ family protein	-
LMJ44_RS07815 (LMJ44_07820)	1675196..1675792	-	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
LMJ44_RS07820 (LMJ44_07825)	1676184..1676354	+	171	WP_013097201.1	general stress protein	-
LMJ44_RS07825 (LMJ44_07830)	1676473..1677390	+	918	WP_023292825.1	DMT family transporter	-
LMJ44_RS07830 (LMJ44_07835)	1677452..1677721	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
LMJ44_RS07835 (LMJ44_07840)	1677728..1678249	+	522	WP_063417750.1	GNAT family N-acetyltransferase	Toxin
LMJ44_RS07840 (LMJ44_07845)	1678323..1679645	-	1323	WP_063417749.1	pyrimidine utilization transport protein G	-
LMJ44_RS07845 (LMJ44_07850)	1679667..1680161	-	495	WP_023292823.1	pyrimidine utilization flavin reductase protein F	-
LMJ44_RS07850 (LMJ44_07855)	1680171..1680761	-	591	WP_063417748.1	malonic semialdehyde reductase	-
LMJ44_RS07855 (LMJ44_07860)	1680771..1681571	-	801	WP_063417747.1	pyrimidine utilization protein D	-
LMJ44_RS07860 (LMJ44_07865)	1681579..1681965	-	387	WP_008499822.1	pyrimidine utilization protein C	-
LMJ44_RS07865 (LMJ44_07870)	1681977..1682666	-	690	WP_063417746.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19656.42 Da
• Isoelectric Point: 7.4313

>T223182 WP_063417750.1 NZ_CP086405:1677728-1678249 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTGENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T223182 NZ_CP118549:c66706-65843 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT223182 WP_008499829.1 NZ_CP086405:1677452-1677721 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT223182 NZ_CP118549:c66980-66708 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: W7PG43