Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1082679..1083299 Replicon chromosome
Accession NZ_CP086405
Organism Enterobacter roggenkampii strain POL1

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag LMJ44_RS05030 Protein ID WP_008499287.1
Coordinates 1082679..1082897 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LMJ44_RS05035 Protein ID WP_008499288.1
Coordinates 1082925..1083299 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMJ44_RS05000 (LMJ44_05005) 1078692..1078952 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
LMJ44_RS05005 (LMJ44_05010) 1078955..1079095 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LMJ44_RS05010 (LMJ44_05015) 1079092..1079802 - 711 WP_063418459.1 GNAT family protein -
LMJ44_RS05015 (LMJ44_05020) 1079904..1081364 + 1461 WP_063418458.1 PLP-dependent aminotransferase family protein -
LMJ44_RS05020 (LMJ44_05025) 1081336..1081803 - 468 WP_008499285.1 YlaC family protein -
LMJ44_RS05025 (LMJ44_05030) 1081921..1082472 - 552 WP_021241634.1 maltose O-acetyltransferase -
LMJ44_RS05030 (LMJ44_05035) 1082679..1082897 - 219 WP_008499287.1 HHA domain-containing protein Toxin
LMJ44_RS05035 (LMJ44_05040) 1082925..1083299 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LMJ44_RS05040 (LMJ44_05045) 1083809..1086955 - 3147 WP_008499289.1 multidrug efflux RND transporter permease subunit AcrB -
LMJ44_RS05045 (LMJ44_05050) 1086978..1088171 - 1194 WP_021241633.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T223181 WP_008499287.1 NZ_CP086405:c1082897-1082679 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T223181 NZ_CP118548:187240-187506 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT223181 WP_008499288.1 NZ_CP086405:c1083299-1082925 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT223181 NZ_CP118548:186977-187240 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References