Detailed information of TA system TA223180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 605687..606263
• Replicon: chromosome
• Accession: NZ_CP086405
• Organism: Enterobacter roggenkampii strain POL1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LMJ44_RS02835
• Protein ID: WP_063418479.1
• Coordinates: 605687..605974 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A156JBA1
• Locus tag: LMJ44_RS02840
• Protein ID: WP_023293359.1
• Coordinates: 605961..606263 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ44_RS02805 (LMJ44_02810)	600700..600816	+	117	Protein_543	amino acid-binding protein	-
LMJ44_RS02810 (LMJ44_02815)	600809..601504	+	696	WP_063418331.1	winged helix-turn-helix domain-containing protein	-
LMJ44_RS02815 (LMJ44_02820)	601650..602120	+	471	WP_021240623.1	MarR family transcriptional regulator	-
LMJ44_RS02820 (LMJ44_02825)	602117..603184	+	1068	WP_088728436.1	HlyD family secretion protein	-
LMJ44_RS02825 (LMJ44_02830)	603174..604250	+	1077	WP_045286439.1	DUF2955 domain-containing protein	-
LMJ44_RS02830 (LMJ44_02835)	604247..605518	-	1272	WP_063418478.1	DUF445 domain-containing protein	-
LMJ44_RS02835 (LMJ44_02840)	605687..605974	+	288	WP_063418479.1	BrnT family toxin	Toxin
LMJ44_RS02840 (LMJ44_02845)	605961..606263	+	303	WP_023293359.1	BrnA antitoxin family protein	Antitoxin
LMJ44_RS02845 (LMJ44_02850)	606294..606932	-	639	WP_063418480.1	LysE family translocator	-
LMJ44_RS02850 (LMJ44_02855)	606980..607723	-	744	WP_063418481.1	AraC family transcriptional regulator	-
LMJ44_RS02855 (LMJ44_02860)	607875..609245	+	1371	WP_058654250.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
LMJ44_RS02860 (LMJ44_02865)	609291..609611	-	321	WP_008501314.1	hypothetical protein	-
LMJ44_RS02865 (LMJ44_02870)	609611..610156	-	546	WP_063418482.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11292.79 Da
• Isoelectric Point: 7.4007

>T223180 WP_063418479.1 NZ_CP086405:605687-605974 [Enterobacter roggenkampii]
MPMEYEWDNNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T223180 NZ_CP086405:605687-605974 [Enterobacter roggenkampii]
ATGCCAATGGAGTATGAGTGGGATAACAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGGGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11369.01 Da
• Isoelectric Point: 10.2483

>AT223180 WP_023293359.1 NZ_CP086405:605961-606263 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT223180 NZ_CP086405:605961-606263 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A156JBA1