Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2975513..2976148 Replicon chromosome
Accession NZ_CP086393
Organism Paenibacillus macerans strain I6

Toxin (Protein)


Gene name mazF Uniprot ID C6J5R6
Locus tag LMZ02_RS13035 Protein ID WP_009226461.1
Coordinates 2975798..2976148 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A369BFZ7
Locus tag LMZ02_RS13030 Protein ID WP_018750966.1
Coordinates 2975513..2975794 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMZ02_RS13010 (LMZ02_13010) 2971093..2971689 + 597 WP_036625202.1 TetR family transcriptional regulator C-terminal domain-containing protein -
LMZ02_RS13015 (LMZ02_13015) 2971717..2972463 - 747 WP_051985653.1 LytTR family DNA-binding domain-containing protein -
LMZ02_RS13020 (LMZ02_13020) 2972755..2973876 + 1122 WP_036625199.1 outer membrane lipoprotein-sorting protein -
LMZ02_RS13025 (LMZ02_13025) 2974083..2975264 + 1182 WP_036625196.1 alanine racemase -
LMZ02_RS13030 (LMZ02_13030) 2975513..2975794 + 282 WP_018750966.1 ribbon-helix-helix protein, CopG family Antitoxin
LMZ02_RS13035 (LMZ02_13035) 2975798..2976148 + 351 WP_009226461.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMZ02_RS13040 (LMZ02_13040) 2976357..2976713 + 357 WP_036625190.1 DUF4190 domain-containing protein -
LMZ02_RS13045 (LMZ02_13045) 2977108..2979288 + 2181 WP_174905532.1 Tex family protein -
LMZ02_RS13050 (LMZ02_13050) 2979673..2979816 - 144 WP_172531700.1 hypothetical protein -
LMZ02_RS13055 (LMZ02_13055) 2980001..2981059 + 1059 WP_036625184.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-46)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12792.78 Da        Isoelectric Point: 5.6195

>T223178 WP_009226461.1 NZ_CP086393:2975798-2976148 [Paenibacillus macerans]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKKVNDSLLISLGLIDF

Download         Length: 351 bp

>T223178 NZ_CP118547:306759-307064 [Stenotrophomonas maltophilia]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCTGGTGATCACCGCTATCTGGCGGATGGCGTTTCTGAATTGCGTATCGATG
CAGGACCCGGTTACCGCGTGTACTACACCCAGCGGGGCAGGCAGTTGGTGATTCTTCTGGTCGGAGGCGACAAGGGTTCG
CAACAGCGCGATATAGAGAAGGCCAAGGAGATTGCGCGTGCCCTGCGAGCATCGAACTGGTTGTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10783.49 Da        Isoelectric Point: 6.7272

>AT223178 WP_018750966.1 NZ_CP086393:2975513-2975794 [Paenibacillus macerans]
MANMHNTKRIMISLPDHLLQEVDGIVAMENSNRSELIRQAMKLYLNERKKRFIRESMQRGYMEMAKINLSMASEAFHAEE
DADITLDRLVSGV

Download         Length: 282 bp

>AT223178 NZ_CP118547:307105-307407 [Stenotrophomonas maltophilia]
ATGGCTATCAAGAAGAAAGTTGAACTCGAAGCATTTGATGTGGCCGAACACCTTCATACACCGAAGGAAATGGCGGCCTA
TCTCGATGCATGCATCGAAGAAAGCGACGGCGATTCGGCATTCATCGCCAAGGCGCTGGGCGATATCGCGCGTGCCCAAG
GGATGAGCAAGGTCGCACGTGCGGCGGGCCTGTCGCGGGAGAGTCTGTATCGTGCCCTGTCTGGCGAGCGCAGCCCTGAT
TTCTCGACCATCCTGAAGGTGACTCGTGCCCTGGGTGTGCGCCTGCATGCCAGCGCTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P3U1R9

Antitoxin

Source ID Structure
AlphaFold DB A0A369BFZ7

References