Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4767715..4768515 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag LM401_RS25095 Protein ID WP_000342449.1
Coordinates 4767988..4768515 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LM401_RS25090 Protein ID WP_001277108.1
Coordinates 4767715..4767981 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS25070 (4763373) 4763373..4764041 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LM401_RS25075 (4764034) 4764034..4765092 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
LM401_RS25080 (4765337) 4765337..4766191 + 855 WP_228636896.1 RNA polymerase sigma factor RpoH -
LM401_RS25085 (4766462) 4766462..4767565 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LM401_RS25090 (4767715) 4767715..4767981 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LM401_RS25095 (4767988) 4767988..4768515 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LM401_RS25100 (4768512) 4768512..4768895 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
LM401_RS25105 (4769319) 4769319..4770428 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LM401_RS25110 (4770476) 4770476..4771402 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LM401_RS25115 (4771399) 4771399..4772676 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
LM401_RS25120 (4772673) 4772673..4773440 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T223177 WP_000342449.1 NZ_CP086391:4767988-4768515 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T223177 NZ_CP118547:c105170-103848 [Stenotrophomonas maltophilia]
ATGGGTACGCTGCAGGTCTGGATGAATGGTGTGGAAGTCGCACTTTGGGATGATGTCCGGAACGGGTCATCCCGGTTGTC
GTATGTCAGCCAGTGGATCAGCTCGCCCCAGTCCCGACCGTTGTCGCTCTCTCTACCCCTGCTCCCAGATGGCGAGAGCC
ATCGGGGCAACGTCGTCAACGACTACTTCGATAACCTTCTTCCCGATAACGTCGCCATACGCAACCGGATCCGCGACCGG
TTCGCCACGCGCGGCAGCGATACGTTCGATCTTCTTGAAGCCATTGGACGGGATTGTGTCGGCGCAGTGCAGCTGTTGCC
GCCTGCGCATCCACCCGAAGACATCCAGCAGATCACGTGCGAGGTGTTGGACGAAGCAGGTGTCGCTACGATCCTGCGTC
AGGTGACGACAGGGAACCTGCCTGGCGCACCCAGCCGTGATGATGCATTCCGCATTTCCATCGCCGGTGCGCAGGAAAAG
ACCGGTCTTCTGCTGCATGCTGGCAAATGGTGCATTCCTACCGGCAACACACCATCCACTCACATCTTCAAGCTGCCGCT
TGGCATTGTTGGCAACATGCGGGCAGACATGCATCAGTCCGTGGAGAATGAATGGCTGTGCATGCATCTGCTTTCCGCCT
TTGATATCCCCGTGGCCGAGACGCAGATGGGCCGCTTCAACGACCAACGTGCTCTCATTGTCGAGCGGTTTGACCGTCGC
CTGTCTGCCGACCGCCGGTGGTGGCTGCGACTGCCACAGGAAGACATGTGTCAGGTCTTTGGTCTGCCGGCTGCCAGAAA
GTATGAATCCGATGGCGGCCCCGGTATCGTTCCGATCATGGATCTGCTTCGACAGTCCGAACACCCCGAGGATCGCGCGA
TCTTCTTCAAGACGCAGATTCTTTTCTGGATGCTGGCCGCAACCGACGGCCATGCCAAGAATTTCAGTGTTCACCTGGAA
GCCGGTGGACGCTTCCGCCTGACGCCCGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCAGGGGACCCAATCAACT
CGATCCACGAAACGCACAGCTCGCCATGGCCGTACATGGCAAGAACCGCCACCGGAAACTGCACCTTGTGCGACGGCGGC
ACTGGAATGAAACCGCGCGCGCCTGCGGCGTTGCCGATGGTGCAGAACCATGGATCAAGACATTGATCGCGGCCGTCGAG
CCCGCCATCCAGCAGGTCACCCGTCAGCTCCCGGATGACTTCCCCTCAAACGTGTCCGAACCGATTTTCAACGGACTTCG
TGCTGCGGCAGCACGCCTTTCCAGCATGGCCCCCGAGGCTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT223177 WP_001277108.1 NZ_CP086391:4767715-4767981 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT223177 NZ_CP118547:c105431-105174 [Stenotrophomonas maltophilia]
ATGGACAGGCCGCTGCACACAGTGAGGACAGCCCAACAACTTGCGCCCCTGATGCGTGCTTTCCGGCGCCAGGCGGGCCT
GAGCCAGGCTCAGCTGGCGGAGCGACTCGGGATCAGCCGCCAGGCAGTGGGCGCGCTGGAACGCGACCCTGCATCGGCAA
GTTTTGAACGCCTGATGCGGATCTGGGCGGTGCTGGGTCTGGAGGTCAGCCTCCAGCAACGAAATCCCCAGGAGAACACG
TCGTCATTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References