Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4746422..4747008 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LM401_RS24970 Protein ID WP_042014162.1
Coordinates 4746422..4746652 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LM401_RS24975 Protein ID WP_000593555.1
Coordinates 4746649..4747008 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS24960 (4742459) 4742459..4745194 + 2736 WP_112910035.1 ribosome-associated ATPase/putative transporter RbbA -
LM401_RS24965 (4745194) 4745194..4746318 + 1125 WP_001314210.1 ABC transporter permease -
LM401_RS24970 (4746422) 4746422..4746652 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LM401_RS24975 (4746649) 4746649..4747008 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LM401_RS24980 (4747128) 4747128..4747529 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LM401_RS24985 (4747535) 4747535..4748341 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LM401_RS24990 (4748338) 4748338..4749102 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LM401_RS24995 (4749102) 4749102..4749935 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LM401_RS25000 (4749932) 4749932..4750876 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T223176 WP_042014162.1 NZ_CP086391:4746422-4746652 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T223176 NZ_CP118545:200658-201008 [Priestia aryabhattai]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT223176 WP_000593555.1 NZ_CP086391:4746649-4747008 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT223176 NZ_CP118545:200372-200653 [Priestia aryabhattai]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References