Detailed information of TA system TA223171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3229461..3230298
• Replicon: chromosome
• Accession: NZ_CP086391
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: LM401_RS17945
• Protein ID: WP_112910017.1
• Coordinates: 3229756..3230298 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LM401_RS17940
• Protein ID: WP_001297137.1
• Coordinates: 3229461..3229772 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS17915 (3224481)	3224481..3225428	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LM401_RS17920 (3225450)	3225450..3227441	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LM401_RS17925 (3227431)	3227431..3228045	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LM401_RS17930 (3228045)	3228045..3228374	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LM401_RS17935 (3228386)	3228386..3229276	+	891	WP_000971336.1	heme o synthase	-
LM401_RS17940 (3229461)	3229461..3229772	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LM401_RS17945 (3229756)	3229756..3230298	+	543	WP_112910017.1	GNAT family N-acetyltransferase	Toxin
LM401_RS17950 (3230354)	3230354..3231289	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
LM401_RS17955 (3231697)	3231697..3233061	+	1365	WP_001000978.1	MFS transporter	-
LM401_RS17960 (3233189)	3233189..3233680	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LM401_RS17965 (3233848)	3233848..3234759	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19833.07 Da
• Isoelectric Point: 8.3395

>T223171 WP_112910017.1 NZ_CP086391:3229756-3230298 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYVRLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T223171 NZ_CP118542:c4460262-4459951 [Salmonella sp. CVCC 1806]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT223171 WP_001297137.1 NZ_CP086391:3229461-3229772 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT223171 NZ_CP118542:c4459950-4459660 [Salmonella sp. CVCC 1806]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3