Detailed information of TA system TA223166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1919074..1919600
• Replicon: chromosome
• Accession: NZ_CP086391
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: LM401_RS11560
• Protein ID: WP_000323025.1
• Coordinates: 1919313..1919600 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: LM401_RS11555
• Protein ID: WP_000534858.1
• Coordinates: 1919074..1919313 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS11525 (1914411)	1914411..1914900	+	490	Protein_1824	class I SAM-dependent methyltransferase	-
LM401_RS11530 (1915378)	1915378..1915797	+	420	Protein_1825	hypothetical protein	-
LM401_RS11535 (1916453)	1916453..1917502	+	1050	Protein_1826	ISNCY family transposase	-
LM401_RS11540 (1917623)	1917623..1917772	+	150	WP_011443592.1	protein YdfW	-
LM401_RS11545 (1918209)	1918209..1918541	-	333	WP_001301033.1	FlxA-like family protein	-
LM401_RS11550 (1918744)	1918744..1919049	-	306	WP_071593935.1	protein YdfV	-
LM401_RS11555 (1919074)	1919074..1919313	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
LM401_RS11560 (1919313)	1919313..1919600	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LM401_RS11565 (1919672)	1919672..1919827	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
LM401_RS11570 (1920044)	1920044..1920295	+	252	WP_001463433.1	protein Rem	-
LM401_RS11575 (1920362)	1920362..1920640	+	279	WP_001515373.1	hypothetical protein	-
LM401_RS11580 (1920642)	1920642..1921691	+	1050	WP_001515372.1	DUF968 domain-containing protein	-
LM401_RS11585 (1921709)	1921709..1922086	+	378	WP_001204787.1	antiterminator Q family protein	-
LM401_RS11590 (1922242)	1922242..1922766	-	525	WP_000780584.1	lipocalin family protein	-
LM401_RS11595 (1922959)	1922959..1923918	+	960	WP_000592549.1	DUF523 and DUF1722 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1901898..1959800	57902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T223166 WP_000323025.1 NZ_CP086391:1919313-1919600 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T223166 NZ_CP118542:3599030-3599158 [Salmonella sp. CVCC 1806]
GTGAAGATTTCGGACGGGTGTATTGTGCTAATGGCGGACGGTAACGAGGTGCAGGAGCTGCGCGAACAGCTTTATCAGGC
TAAACGGGTGGTTAAGGGGGGTGAAGGATGTGCTGGTTTAGTGGACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT223166 WP_000534858.1 NZ_CP086391:1919074-1919313 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT223166 NZ_CP118542:c3599108-3598756 [Salmonella sp. CVCC 1806]
CCTGATAAAGCTGTTCGCGCAGCTCCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCGTCCGAAATCTTCACC
GTCACGCCGCTCCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCCGGGGATTTGTGAGTAACAGGTGGTCATGC
AGGTTTCGCTGTTGATGTGGCGGACGCTGGCGCCGGAGGTATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCC
GGATGCTGGCTGATTGTGGAGCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCCCCCTCTCCCCGGCCA
GTGATGAGCAACCGGCTACCGGGGCCGTATTTT

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8