Detailed information of TA system TA223156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 961487..962112
• Replicon: chromosome
• Accession: NZ_CP086391
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LM401_RS06900
• Protein ID: WP_000911330.1
• Coordinates: 961714..962112 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LM401_RS06895
• Protein ID: WP_000450524.1
• Coordinates: 961487..961714 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS06870 (957290)	957290..957760	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LM401_RS06875 (957760)	957760..958332	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LM401_RS06880 (958478)	958478..959356	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LM401_RS06885 (959373)	959373..960407	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LM401_RS06890 (960620)	960620..961333	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LM401_RS06895 (961487)	961487..961714	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LM401_RS06900 (961714)	961714..962112	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LM401_RS06905 (962259)	962259..963122	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
LM401_RS06910 (963137)	963137..965152	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
LM401_RS06915 (965226)	965226..965924	+	699	WP_000679823.1	esterase	-
LM401_RS06920 (966034)	966034..966234	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T223156 WP_000911330.1 NZ_CP086391:961714-962112 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T223156 NZ_CP118541:468498-468848 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT223156 WP_000450524.1 NZ_CP086391:961487-961714 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT223156 NZ_CP118541:468211-468492 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33