Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 671922..672505 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LM401_RS05490 Protein ID WP_112910055.1
Coordinates 672170..672505 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LM401_RS05485 Protein ID WP_000581937.1
Coordinates 671922..672170 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS05475 (668261) 668261..669562 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
LM401_RS05480 (669610) 669610..671844 + 2235 WP_000226815.1 GTP pyrophosphokinase -
LM401_RS05485 (671922) 671922..672170 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LM401_RS05490 (672170) 672170..672505 + 336 WP_112910055.1 endoribonuclease MazF Toxin
LM401_RS05495 (672576) 672576..673367 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
LM401_RS05500 (673595) 673595..675232 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LM401_RS05505 (675320) 675320..676618 + 1299 WP_000036723.1 phosphopyruvate hydratase -
LM401_RS05510 (676674) 676674..677036 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12109.00 Da        Isoelectric Point: 8.4921

>T223155 WP_112910055.1 NZ_CP086391:672170-672505 [Escherichia coli]
MVSRNVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T223155 NZ_CP118540:c36427-36137 [Xylella fastidiosa]
GTGGAACGATATTTCGAGTGGGACGACAAGAAGGCGGAAAGCAACTTCCGTAAGCACGGCATCCGGTTCGATGAAGCTGC
GCTTGCCTTCGACGACCCGTTTGCAGTGTCGGAACAAAACCGTATCGAAAATGGGGAGCAGCGTTGGCAAACCATCGGAA
TGGCCGGTGGGTGCCTGCTGCTGCTGGTGGCGCACACAGTGCACTTTGAAGATGATGGCATCGAGGTTGTACGAATCATC
AGTGCCCGTCGCGTGGACCGAAAGGAACGGAGGCGTTATGAACATGGTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT223155 WP_000581937.1 NZ_CP086391:671922-672170 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT223155 NZ_CP118540:c36150-35845 [Xylella fastidiosa]
ATGAACATGGTTAGGTACAAGCAGAGCGAGTTGCCGCCGCTGACCGAAGAACGTAAGGCAGAACTCAAGGCGCTAGCAAA
GAAGCCAGATAGCGAAATCGACTACAGCGATATTCCACCGTTGGATGAATCGTTTTGGGCGCGGGCAGTACCCAACCCAT
TCTTTAAGCCGGTCAAGACACATGCTTCTGTGCGTATTGATTCGGATGTGTTGGCTTGGCTCAAGAGTCAAGGCAAGGGC
TACCAGACCCGTATGAATGCAATCTTGCGAGAAGCCATGCTTCGCTCGCTGCACCAGCAAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References