Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 534326..534980 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LM401_RS04865 Protein ID WP_000244772.1
Coordinates 534573..534980 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LM401_RS04860 Protein ID WP_000354046.1
Coordinates 534326..534592 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS04835 (529614) 529614..530357 + 744 WP_000951964.1 SDR family oxidoreductase -
LM401_RS04840 (530414) 530414..531847 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LM401_RS04845 (531892) 531892..532203 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
LM401_RS04850 (532367) 532367..533026 + 660 WP_000250274.1 hemolysin III family protein -
LM401_RS04855 (533103) 533103..534083 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LM401_RS04860 (534326) 534326..534592 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LM401_RS04865 (534573) 534573..534980 + 408 WP_000244772.1 protein YgfX Toxin
LM401_RS04870 (535020) 535020..535541 - 522 WP_001055874.1 flavodoxin FldB -
LM401_RS04875 (535653) 535653..536549 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LM401_RS04880 (536574) 536574..537284 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LM401_RS04885 (537290) 537290..539023 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T223154 WP_000244772.1 NZ_CP086391:534573-534980 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223154 NZ_CP118539:c2531055-2530756 [Xylella fastidiosa]
ATGACCTACACGCTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCGGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223154 WP_000354046.1 NZ_CP086391:534326-534592 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223154 NZ_CP118539:c2530752-2530444 [Xylella fastidiosa]
GTGACCATAACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTTGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCCCTTCGGCCTGGTAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References