Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 96488..98111 Replicon plasmid p1585m1_A
Accession NZ_CP086389
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LM401_RS01715 Protein ID WP_000088046.1
Coordinates 96786..98111 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LM401_RS01710 Protein ID WP_024136327.1
Coordinates 96488..96781 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS01685 (LM401_01685) 92360..93601 + 1242 WP_001053338.1 VWA domain-containing protein -
LM401_RS25675 93692..93823 + 132 WP_000374059.1 hypothetical protein -
LM401_RS01690 (LM401_01690) 94164..94445 - 282 WP_000077926.1 hypothetical protein -
LM401_RS01695 (LM401_01695) 94495..94686 - 192 WP_000398480.1 hypothetical protein -
LM401_RS01700 (LM401_01700) 94778..95119 - 342 WP_001151575.1 hypothetical protein -
LM401_RS01705 (LM401_01705) 95492..95884 + 393 WP_000341066.1 hypothetical protein -
LM401_RS01710 (LM401_01710) 96488..96781 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LM401_RS01715 (LM401_01715) 96786..98111 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LM401_RS01720 (LM401_01720) 98172..98378 + 207 WP_000134171.1 hypothetical protein -
LM401_RS01725 (LM401_01725) 98480..98890 + 411 WP_000985911.1 hypothetical protein -
LM401_RS01730 (LM401_01730) 98903..99718 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
LM401_RS01735 (LM401_01735) 99972..100397 + 426 WP_001043843.1 hypothetical protein -
LM401_RS01740 (LM401_01740) 100946..101254 + 309 WP_001224687.1 hypothetical protein -
LM401_RS01745 (LM401_01745) 101270..102127 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / sul1 / qacE / catB3 / blaIMP-4 - 1..142329 142329


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T223152 WP_000088046.1 NZ_CP086389:96786-98111 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T223152 NZ_CP118539:c1597625-1597371 [Xylella fastidiosa]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGACTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCTCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT223152 WP_024136327.1 NZ_CP086389:96488-96781 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT223152 NZ_CP118539:c1597374-1597039 [Xylella fastidiosa]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGATAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References