Detailed information of TA system TA223151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 63098..63741
• Replicon: plasmid p1585m1_B
• Accession: NZ_CP086388
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D7Y5A6
• Locus tag: LM401_RS00965
• Protein ID: WP_001044765.1
• Coordinates: 63098..63514 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LM401_RS00970
• Protein ID: WP_001261282.1
• Coordinates: 63511..63741 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS00935 (LM401_00935)	58655..59044	-	390	WP_003980690.1	hypothetical protein	-
LM401_RS00940 (LM401_00940)	59301..59513	+	213	WP_032187681.1	hypothetical protein	-
LM401_RS00945 (LM401_00945)	59626..61857	+	2232	WP_124062889.1	porphyrin biosynthesis protein	-
LM401_RS00950 (LM401_00950)	61953..62120	+	168	WP_229014370.1	hypothetical protein	-
LM401_RS00955 (LM401_00955)	62183..62887	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LM401_RS00960 (LM401_00960)	62941..63024	-	84	Protein_77	hypothetical protein	-
LM401_RS00965 (LM401_00965)	63098..63514	-	417	WP_001044765.1	type II toxin-antitoxin system VapC family toxin	Toxin
LM401_RS00970 (LM401_00970)	63511..63741	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LM401_RS00975 (LM401_00975)	63698..64120	+	423	WP_177952108.1	hypothetical protein	-
LM401_RS00980 (LM401_00980)	64304..64666	+	363	WP_000533745.1	hypothetical protein	-
LM401_RS00985 (LM401_00985)	64684..65493	+	810	WP_000654269.1	hypothetical protein	-
LM401_RS00990 (LM401_00990)	66058..67731	+	1674	WP_000026497.1	hypothetical protein	-
LM401_RS00995 (LM401_00995)	67922..68228	+	307	Protein_84	type II toxin-antitoxin system ParD family antitoxin	-
LM401_RS01000 (LM401_01000)	68215..68545	+	331	Protein_85	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / catB3 / blaIMP-4	-	1..113047	113047
	-	flank	IS/Tn	-	-	62183..62887	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15148.60 Da
• Isoelectric Point: 7.1082

>T223151 WP_001044765.1 NZ_CP086388:c63514-63098 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T223151 NZ_CP118539:1318746-1319027 [Xylella fastidiosa]
ATGCGGAGGATTAGCCAGACTGGCCAATTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTGATACACGTCTTGGAAGCCTTGACCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT223151 WP_001261282.1 NZ_CP086388:c63741-63511 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT223151 NZ_CP118539:1318496-1318759 [Xylella fastidiosa]
ATGTCTGCAAACGCAGTTGTCCGTGCCCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTGCTGGCGACGATGGG
CCTAAGCGTGTCTGATGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGAAACTACCATCGCAGCCATGCGTGAAGCCCGTGCTGGTCACCTCAAGAGCTTTGATAGTATTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A2H9AY90

Antitoxin

• AlphaFold DB: A0A0H3GZG3