Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 37605..38403 Replicon plasmid p1585m1_B
Accession NZ_CP086388
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name ataT Uniprot ID A0A2L1KTV6
Locus tag LM401_RS00815 Protein ID WP_023135659.1
Coordinates 37605..38126 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag LM401_RS00820 Protein ID WP_001711191.1
Coordinates 38134..38403 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS00790 (LM401_00790) 32884..33525 + 642 WP_023135664.1 hypothetical protein -
LM401_RS00795 (LM401_00795) 33528..34784 + 1257 WP_023135663.1 phage terminase large subunit -
LM401_RS00800 (LM401_00800) 34818..36392 + 1575 Protein_46 hypothetical protein -
LM401_RS00805 (LM401_00805) 36415..37182 + 768 WP_023135661.1 hypothetical protein -
LM401_RS00810 (LM401_00810) 37185..37436 + 252 WP_023135660.1 hypothetical protein -
LM401_RS00815 (LM401_00815) 37605..38126 - 522 WP_023135659.1 GNAT family N-acetyltransferase Toxin
LM401_RS00820 (LM401_00820) 38134..38403 - 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
LM401_RS00825 (LM401_00825) 38723..39388 + 666 WP_023135658.1 AAA family ATPase -
LM401_RS00830 (LM401_00830) 39394..39747 + 354 WP_161953766.1 hypothetical protein -
LM401_RS00835 (LM401_00835) 39800..40567 - 768 WP_001711193.1 hypothetical protein -
LM401_RS00840 (LM401_00840) 40850..41575 + 726 WP_023135656.1 hypothetical protein -
LM401_RS00845 (LM401_00845) 41636..42976 + 1341 WP_023135655.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / catB3 / blaIMP-4 - 1..113047 113047


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19382.22 Da        Isoelectric Point: 8.2535

>T223150 WP_023135659.1 NZ_CP086388:c38126-37605 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T223150 NZ_CP118539:c1228167-1227556 [Xylella fastidiosa]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGGGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGTGGATTGGC
TAGTATCATCCGCGAAGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT223150 WP_001711191.1 NZ_CP086388:c38403-38134 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp

>AT223150 NZ_CP118539:c1228352-1228164 [Xylella fastidiosa]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1KTV6

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References