Detailed information of TA system TA223145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 115647..116308
• Replicon: plasmid pRadio2
• Accession: NZ_CP086382
• Organism: Deinococcus radiophilus strain ATCC 27603

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMT64_RS12755
• Protein ID: WP_126353636.1
• Coordinates: 115889..116308 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LMT64_RS12750
• Protein ID: WP_126353638.1
• Coordinates: 115647..115889 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMT64_RS12720 (LMT64_12715)	111199..111486	+	288	WP_229253533.1	transposase	-
LMT64_RS12725 (LMT64_12720)	111501..112358	+	858	WP_229253570.1	integrase core domain-containing protein	-
LMT64_RS12730 (LMT64_12725)	112303..113490	+	1188	WP_126353643.1	TIGR02221 family CRISPR-associated protein	-
LMT64_RS12735 (LMT64_12730)	113603..114571	-	969	WP_229253521.1	site-specific integrase	-
LMT64_RS12740 (LMT64_12735)	114983..115225	+	243	WP_126353641.1	hypothetical protein	-
LMT64_RS12745 (LMT64_12740)	115222..115557	+	336	WP_126353639.1	type II toxin-antitoxin system PemK/MazF family toxin	-
LMT64_RS12750 (LMT64_12745)	115647..115889	+	243	WP_126353638.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LMT64_RS12755 (LMT64_12750)	115889..116308	+	420	WP_126353636.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMT64_RS12760 (LMT64_12755)	116289..116927	-	639	WP_170166071.1	ATP-dependent helicase	-
LMT64_RS12765 (LMT64_12760)	117362..117649	+	288	WP_229253522.1	transposase	-
LMT64_RS12770 (LMT64_12765)	117652..118521	+	870	WP_229253523.1	integrase core domain-containing protein	-
LMT64_RS12775 (LMT64_12770)	119064..119834	-	771	WP_126352757.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..224843	224843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15453.75 Da
• Isoelectric Point: 5.8833

>T223145 WP_126353636.1 NZ_CP086382:115889-116308 [Deinococcus radiophilus]
MYLLDTNVISELRKVKSGRANEGVVAWSQTVTTAELYLSVITVQELELGVLLKERRDLQQGAVLRKWLDGQVLPAFAGRI
LPITLEIARRTANLNVPQQRAFYDSLIAATALEHGYTLVTRNEADMQGTGVEIVNPFQS

Nucleotide

• Length: 420 bp

>T223145 NZ_CP118539:457920-458222 [Xylella fastidiosa]
ATGGAGAAAGGCACATCTCACTATAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTCGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9002.12 Da
• Isoelectric Point: 4.4929

>AT223145 WP_126353638.1 NZ_CP086382:115647-115889 [Deinococcus radiophilus]
MTTISAREFNQQVSKVRKAASKEPVFITERGEPKHVLLSIEAYEALTGSQRNIADLLALDEDIDFELPQRSELTRAAELD

Nucleotide

• Length: 243 bp

>AT223145 NZ_CP118539:457557-457832 [Xylella fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG