Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-vapB/MazF-YefM
Location 115222..115889 Replicon plasmid pRadio2
Accession NZ_CP086382
Organism Deinococcus radiophilus strain ATCC 27603

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LMT64_RS12745 Protein ID WP_126353639.1
Coordinates 115222..115557 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag LMT64_RS12750 Protein ID WP_126353638.1
Coordinates 115647..115889 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMT64_RS12720 (LMT64_12715) 111199..111486 + 288 WP_229253533.1 transposase -
LMT64_RS12725 (LMT64_12720) 111501..112358 + 858 WP_229253570.1 integrase core domain-containing protein -
LMT64_RS12730 (LMT64_12725) 112303..113490 + 1188 WP_126353643.1 TIGR02221 family CRISPR-associated protein -
LMT64_RS12735 (LMT64_12730) 113603..114571 - 969 WP_229253521.1 site-specific integrase -
LMT64_RS12740 (LMT64_12735) 114983..115225 + 243 WP_126353641.1 hypothetical protein -
LMT64_RS12745 (LMT64_12740) 115222..115557 + 336 WP_126353639.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMT64_RS12750 (LMT64_12745) 115647..115889 + 243 WP_126353638.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LMT64_RS12755 (LMT64_12750) 115889..116308 + 420 WP_126353636.1 type II toxin-antitoxin system VapC family toxin -
LMT64_RS12760 (LMT64_12755) 116289..116927 - 639 WP_170166071.1 ATP-dependent helicase -
LMT64_RS12765 (LMT64_12760) 117362..117649 + 288 WP_229253522.1 transposase -
LMT64_RS12770 (LMT64_12765) 117652..118521 + 870 WP_229253523.1 integrase core domain-containing protein -
LMT64_RS12775 (LMT64_12770) 119064..119834 - 771 WP_126352757.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..224843 224843


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-107)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12310.16 Da        Isoelectric Point: 8.9343

>T223144 WP_126353639.1 NZ_CP086382:115222-115557 [Deinococcus radiophilus]
MKRGDIYQVDFEPSVQGEPARSRPAVILTNDLANEALPHLVVAPITSNVSREYPFDVMLPVGTCGLPETSRVQLNYVRGL
NRNKFGAYLGSLTRAQMSELDRKLKLHLGLS

Download         Length: 336 bp

>T223144 NZ_CP118539:457265-457546 [Xylella fastidiosa]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9002.12 Da        Isoelectric Point: 4.4929

>AT223144 WP_126353638.1 NZ_CP086382:115647-115889 [Deinococcus radiophilus]
MTTISAREFNQQVSKVRKAASKEPVFITERGEPKHVLLSIEAYEALTGSQRNIADLLALDEDIDFELPQRSELTRAAELD

Download         Length: 243 bp

>AT223144 NZ_CP118539:457557-457832 [Xylella fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References