Detailed information of TA system TA223142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1558833..1559471
• Replicon: chromosome
• Accession: NZ_CP086373
• Organism: Paenibacillus polymyxa strain MEZ6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: LMH85_RS06605
• Protein ID: WP_007429335.1
• Coordinates: 1559121..1559471 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: LMH85_RS06600
• Protein ID: WP_007429334.1
• Coordinates: 1558833..1559114 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH85_RS06580 (LMH85_06580)	1553850..1554821	-	972	WP_167440839.1	ABC transporter substrate-binding protein	-
LMH85_RS06585 (LMH85_06585)	1555056..1555688	+	633	WP_031463107.1	LysE family transporter	-
LMH85_RS06590 (LMH85_06590)	1555799..1557076	+	1278	WP_031463108.1	outer membrane lipoprotein-sorting protein	-
LMH85_RS06595 (LMH85_06595)	1557343..1558539	+	1197	WP_031463109.1	alanine racemase	-
LMH85_RS06600 (LMH85_06600)	1558833..1559114	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
LMH85_RS06605 (LMH85_06605)	1559121..1559471	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LMH85_RS06610 (LMH85_06610)	1559650..1561872	+	2223	WP_017426526.1	Tex family protein	-
LMH85_RS06615 (LMH85_06615)	1561971..1562105	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
LMH85_RS06620 (LMH85_06620)	1562248..1562760	+	513	WP_016821162.1	SprT family protein	-
LMH85_RS06635 (LMH85_06635)	1563139..1564014	-	876	WP_031463111.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T223142 WP_007429335.1 NZ_CP086373:1559121-1559471 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T223142 NZ_CP118537:4535282-4535593 [Salmonella enterica subsp. enterica serovar Typhi]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT223142 WP_007429334.1 NZ_CP086373:1558833-1559114 [Paenibacillus polymyxa]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT223142 NZ_CP118537:4535590-4536036 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0