Detailed information of TA system TA223127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3924951..3925576
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: LLE50_RS17690
• Protein ID: WP_005976087.1
• Coordinates: 3924951..3925154 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: C6DB73
• Locus tag: LLE50_RS17695
• Protein ID: WP_005976089.1
• Coordinates: 3925208..3925576 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS17660 (LLE50_17650)	3920412..3920750	+	339	WP_002208627.1	P-II family nitrogen regulator	-
LLE50_RS17665 (LLE50_17655)	3920790..3922076	+	1287	WP_161973278.1	ammonium transporter AmtB	-
LLE50_RS17670 (LLE50_17660)	3922211..3923074	-	864	WP_130622068.1	acyl-CoA thioesterase II	-
LLE50_RS17675 (LLE50_17665)	3923287..3923868	+	582	WP_103941783.1	YbaY family lipoprotein	-
LLE50_RS17680 (LLE50_17670)	3923888..3924208	-	321	WP_015839360.1	MGMT family protein	-
LLE50_RS17690 (LLE50_17680)	3924951..3925154	-	204	WP_005976087.1	HHA domain-containing protein	Toxin
LLE50_RS17695 (LLE50_17685)	3925208..3925576	-	369	WP_005976089.1	Hha toxicity modulator TomB	Antitoxin
LLE50_RS17700 (LLE50_17690)	3926174..3926317	-	144	WP_005976091.1	type B 50S ribosomal protein L36	-
LLE50_RS17705 (LLE50_17695)	3926334..3926582	-	249	WP_039506474.1	type B 50S ribosomal protein L31	-
LLE50_RS17710 (LLE50_17700)	3926809..3928002	-	1194	WP_103863058.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3926809..3928002	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T223127 WP_005976087.1 NZ_CP086369:c3925154-3924951 [Pectobacterium versatile]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T223127 NZ_CP118536:c4109890-4109402 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14032.77 Da
• Isoelectric Point: 4.4787

>AT223127 WP_005976089.1 NZ_CP086369:c3925576-3925208 [Pectobacterium versatile]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Nucleotide

• Length: 369 bp

>AT223127 NZ_CP118536:c4110180-4109887 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGTCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACTGCACAGACTTTGTGCTAGAAGCTGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGATCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0

Antitoxin

• AlphaFold DB: A0A1D7Z3G5