Detailed information of TA system TA223126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3667840..3668366
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LLE50_RS16605
• Protein ID: WP_130621816.1
• Coordinates: 3667840..3668145 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3R8NIS7
• Locus tag: LLE50_RS16610
• Protein ID: WP_038917755.1
• Coordinates: 3668148..3668366 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS16580 (LLE50_16570)	3663263..3663433	+	171	Protein_3233	helix-turn-helix domain-containing protein	-
LLE50_RS16585 (LLE50_16575)	3663772..3664065	+	294	WP_130621812.1	DUF1778 domain-containing protein	-
LLE50_RS16590 (LLE50_16580)	3664062..3664556	+	495	WP_130621813.1	GNAT family N-acetyltransferase	-
LLE50_RS16595 (LLE50_16585)	3664982..3666712	+	1731	WP_130621814.1	AAA family ATPase	-
LLE50_RS16600 (LLE50_16590)	3666798..3667640	+	843	WP_130621815.1	DNA adenine methylase	-
LLE50_RS16605 (LLE50_16595)	3667840..3668145	-	306	WP_130621816.1	type II toxin-antitoxin system toxin CcdB	Toxin
LLE50_RS16610 (LLE50_16600)	3668148..3668366	-	219	WP_038917755.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LLE50_RS16615 (LLE50_16605)	3668425..3669168	-	744	WP_130621817.1	MobC family replication-relaxation protein	-
LLE50_RS16620 (LLE50_16610)	3669305..3669466	+	162	WP_161973602.1	hypothetical protein	-
LLE50_RS16625 (LLE50_16615)	3670066..3670407	-	342	WP_130621818.1	hypothetical protein	-
LLE50_RS16630 (LLE50_16620)	3670593..3670787	+	195	WP_015854710.1	YlcI/YnfO family protein	-
LLE50_RS16635 (LLE50_16625)	3670893..3672152	-	1260	WP_130621819.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3648153..3672248	24095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11678.62 Da
• Isoelectric Point: 5.1200

>T223126 WP_130621816.1 NZ_CP086369:c3668145-3667840 [Pectobacterium versatile]
MQFIVYGYKRASHYKMFVDVQSDIVETPKRRMVIPIIESHHLSEKVNKTLFPLIRIDGEGYRLMTMELSSVPVEVIGEVI
ADLGDYSDEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T223126 NZ_CP118536:c3992566-3992282 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8229.18 Da
• Isoelectric Point: 7.2041

>AT223126 WP_038917755.1 NZ_CP086369:c3668366-3668148 [Pectobacterium versatile]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIRAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT223126 NZ_CP118536:c3992798-3992556 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A3R8NIS7